Ip3-mediated Calcium
Ip3-mediated Calcium - Related Videos
01:11
IP3/DAG Signaling Pathway
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2023
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...
03:43
An Imaging Technique to Study the Calcium Response in Bacteria-Infected Cells
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2025
This video demonstrates a technique for detecting local and global calcium ion response upon Shigella infection of human epithelial cells. Shigella invades host cells and releases invasion effectors, impacting intracellular calcium levels. The amplitude and frequency of the local and global cytosolic calcium are studied using a fluorescent indicator.
03:54
Monitoring B Cell Activation with Antigenic Liposomes using a Calcium-Flux Assay
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2025
This video showcases the assessment of B cell activation through the utilization of antigenic liposomes. B cells treated with calcium-sensitive Indo-1 dye are distinguished using flow cytometry, and their violet-to-blue fluorescence ratio is analyzed. As the cells encounter antigenic liposomes, it triggers B cell activation through calcium influx, progressively enhancing the violet-to-blue fluorescence signal ratio over time.
13:40
Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms
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Cited by 17 •
2011
The endoplasmic reticulum plays a key role in protein biogenesis and in calcium homeostasis. We have established an experimental system that allows us to address the role of Ca2+ leak channels and to characterize their putative regulatory mechanisms. This system involves siRNA mediated gene silencing and live cell Ca2+ imaging.
08:43
Calcium Imaging in Neurons
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2023
Calcium ions play an integral role in neuron function: They act as intracellular signals that can elicit responses such as altered gene expression and neurotransmitter release from synaptic vesicles. Within the cell, calcium concentration is highly dynamic due to the presence of pumps that selectively transport these ions in response to a variety of signals. Calcium imaging takes advantage of intracellular calcium flux to directly visualize calcium signaling in living neurons.This video begins...
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The signal is limited by two opposing processes: calcium can be buffered within the cell, and pumps can return it to intracellular stores. This recovery reduces cytosolic Ca2+ after the initial increase, allowing the cell to produce controlled, short-lived signals instead of maintaining continuous activation. Such timing helps coordinate responses without losing signaling precision.
Phospholipase C provides the biochemical link between receptor activation and calcium release. After an extracellular signal activates a cell-surface receptor, the enzyme cleaves membrane PIP2, producing IP3 and diacylglycerol. IP3 then carries the signal to receptors on the endoplasmic reticulum, converting information at the cell surface into a change in cytosolic Ca2+.
The endoplasmic reticulum serves as the intracellular source from which calcium is released during this pathway. IP3 binds receptors located on its membrane, causing calcium channels to open and increasing cytosolic Ca2+. Because calcium can later be returned to intracellular stores, this arrangement supports a regulated rise and recovery rather than an irreversible change in cellular calcium.
The pathway passes information through several linked stages: a cell-surface receptor detects the extracellular signal, phospholipase C generates IP3 from membrane PIP2, and IP3 opens endoplasmic-reticulum calcium channels. The resulting cytosolic Ca2+ increase acts as an intracellular signal that can influence contraction, secretion, metabolism, gene expression, or cell proliferation.
Changes in cytosolic Ca2+ produced through this pathway can coordinate muscle contraction, secretion, metabolism, gene expression, and cell proliferation. These outcomes show that the same general signaling framework can influence immediate cellular activities as well as longer-term changes in cell behavior. The eventual response depends on how the calcium signal is integrated within the cell.
IP3-mediated calcium signaling links events outside the cell with regulated intracellular responses, making it a central subject for studying cellular communication. Its relevance to disease research follows from the wide range of processes it coordinates, including proliferation, metabolism, secretion, contraction, and gene expression. Examining the pathway therefore helps connect signaling changes with altered cellular behavior.