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July 07, 2011
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The endoplasmic reticulum plays a key role in protein biogenesis and in calcium homeostasis. We have established an experimental system that allows us to address the role of Ca2+ leak channels and to characterize their putative regulatory mechanisms. This system involves siRNA mediated gene silencing and live cell Ca2+ imaging.
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Lang, S., Schäuble, N., Cavalié, A., Zimmermann, R. Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms. J. Vis. Exp. (53), e2730, doi:10.3791/2730 (2011).
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