Larval Feeding

Larval feeding is the process by which immature animals obtain and use nutrients needed for growth, development, and survival, making it a key stage in biology and ecology. Depending on the species, larvae may absorb yolk reserves or capture external food such as plankton, using specialized mouthparts and digestive systems to ingest, break down, and assimilate nutrients; feeding behavior often responds to light, movement, chemical cues, and prey availability. Studying larval feeding helps explain recruitment, metamorphosis, population success, and interactions within aquatic and terrestrial food webs, while informing aquaculture practices, wildlife conservation, and research on development.

Larval Feeding - Related Videos

Research

JoVE Journal - Biology

High-fat Feeding Paradigm for Larval Zebrafish: Feeding, Live Imaging, and Quantification of Food Intake

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Cited by 19 •

2016

Zebrafish are emerging as a valuable model of dietary lipid processing and metabolic disease. Described are protocols of lipid-rich larval feeds, live imaging of dietary fluorescent lipid analogs, and quantification of food intake. These techniques can be applied to a variety of screening, imaging, and hypothesis driven inquiry techniques.

Functional Analysis of the Larval Feeding Circuit in Drosophila

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Cited by 22 •

2013

The feeding circuit in Drosophila melanogaster larvae serves a simple yet powerful model that allows changes in feeding rate to be correlated with alterations in the stomatogastric neural circuitry. This circuit is composed of central serotonergic neurons that send projections to the mouth hooks as well as the foregut.

Feeding Test: Assessment of Feeding Preferences in Drosophila melanogaster

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2023

This video describes a feeding assay for fruit flies in which colored food is used to monitor feeding and the effect added compounds may have on feeding activity. The example protocol tests the effects of several endocrine disrupting chemicals (EDC).

Dissection of Larval Zebrafish Gonadal Tissue

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Cited by 6 •

2017

Here, we present a protocol for isolating gonadal tissue of larval zebrafish, which will facilitate investigations of zebrafish sex differentiation and maintenance.

Education

JoVE Science Education - Basic Biology

Drosophila Larval IHC

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2023

Immunohistochemistry (IHC) is a technique used to visualize the presence and location of proteins within tissues. Drosophila larvae are particularly amenable to IHC because of the ease with which they can be processed for staining. Additionally, the larvae are transparent, meaning that some tissues can be visualized without the need for dissection. In IHC, proteins are ultimately detected with antibodies that specifically bind to “epitopes” within the protein of interest. In order to preserve...

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