Live Cell Yeast Culture

Live cell yeast culture is the laboratory growth and maintenance of viable yeast cells under controlled conditions, enabling researchers to study cellular physiology, genetics, and metabolism. Yeast cells reproduce primarily by budding when supplied with suitable nutrients, water, temperature, and aeration, while sterile technique helps prevent contamination and preserve reliable cultures. These cultures support experiments in molecular and cell biology, including gene function studies, fermentation research, stress-response analysis, and production of recombinant proteins. Because yeast shares many conserved cellular pathways with other eukaryotes, live cell culture provides a practical model for investigating fundamental biological processes and evaluating experimental treatments.

Live Cell Yeast Culture - Related Videos

Research

JoVE Journal - Bioengineering

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

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Cited by 46 •

2013

We describe the use of two ratiometric, genetically encoded biosensors, which are based on GFP, to monitor mitochondrial redox state and ATP levels at subcellular resolution in living yeast cells.

Research

JoVE Journal - Biology
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Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast

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Cited by 11 •

2012

The fission yeast, Schizosaccharomyces pombe, is a good model system to study basic cellular processes. Here we describe a method to perform quantitative live cell analysis of fission yeast. In this particular experiment we focus on organisation of the genome within the cell nucleus, but the method can also be used to study cytosolic factors.

A Rapid Technique for the Visualization of Live Immobilized Yeast Cells

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Cited by 4 •

2006

A rapid technique for the visualization of growing immobilized yeast cells, here applied to fluorescent reporters at the silent mating loci HML and...

Yeast Two-Hybrid Assay to Determine Protein Self-Association in Yeast Cells

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2025

This video demonstrates the yeast two-hybrid assay to detect specific protein self-interactions. In this assay, the protein of interest is tagged to two domains of a transcription factor that encodes the β-galactosidase enzyme. When the two proteins interact, this causes transcription and translation to form the enzyme, which is then detected using a biochemical assay.

Purification of Mitochondria from Yeast Cells

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Cited by 85 •

2009

We describe a rapid and effective method for purification of mitochondria from the yeast Saccharomyces cerevisiae. This method enables the high-yield isolation of pure mitochondria that are essentially free of contamination by other organelles and retain their structural and functional integrity after their purification.

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