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A Rapid Technique for the Visualization of Live Immobilized Yeast Cells
A Rapid Technique for the Visualization of Live Immobilized Yeast Cells
JoVE Journal
Biology
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JoVE Journal Biology
A Rapid Technique for the Visualization of Live Immobilized Yeast Cells

A Rapid Technique for the Visualization of Live Immobilized Yeast Cells

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02:54 min

November 09, 2006

DOI:

02:54 min
November 09, 2006

10406 Views
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Transcript

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The aim of the Experiment is to be able to follow a population of providing sir one cells under the microscope so that I can see how the epigenetics states change how often the epigene states change with each, with each successive division of the cells, take a long cover slip, take about seven microliters of cells, place on the long cover slip and use a com, a synthetic media plate. Take a razor blade and then make cuts into the agar. So take an agar block, lift it up and place over the cells.

Okay, for short term experiments, up to three hours. This by itself is fine. The cells will be immobilized for longer term experiments, I add li liquid medium to the top of the agar pad, and then put a glass slide over top of the whole thing.

The media will provide us like a seal between the two. The, the slide is solely to prevent evaporation from the top of the of the agar block. So with this setup, the egger block will remain moist and the cells will continue dividing for up to eight, at least up to eight hours.

Summary

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A rapid technique for the visualization of growing immobilized yeast cells, here applied to fluorescent reporters at the silent mating loci HML and HMR

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