Mast Cell Mediated Angiogenesis
Mast Cell Mediated Angiogenesis - Related Videos
09:07
Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice
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Cited by 21 •
2015
We describe a method for the generation of in vitro derived mast cells, their engraftment into mast cell-deficient mice, and the analysis of the phenotype, numbers and distribution of engrafted mast cells at different anatomical sites. This protocol can be used to assess the functions of mast cells in vivo.
16:01
Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
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Cited by 9 •
2015
The goal of the present protocol was to develop a method that will allow functional genomic analyses of mast cell secretion. The protocol is based on quantitative assessment of the release of a fluorescent reporter gene cotrasfected with the gene of interest and real time analyses of the secretory granule's morphology.
18:30
Rat Mesentery Angiogenesis Assay
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Cited by 9 •
2011
Normal adult vascularized mammalian tissue that lacks physiologic angiogenesis and that has not been exposed to surgical intervention is used to study: (i) the initiation and development of angiogenesis following intraperitoneal administration of test agents; and (ii) modification of angiogenesis following systemic administration of selected test agents.
03:34
An In Vitro Technique to Visualize Exocytosis of Secretory Granules in Mast Cells
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2025
This video demonstrates a technique to visualize exocytosis in mast cells. The cells are incubated with FITC-dextran — a fluorescent reporter — which is taken up by cells inside secretory granules (SGs), where the acidic pH quenches its fluorescence. Upon stimulation of granule exocytosis, the medium's higher pH induces reporter fluorescence inside the fused SGs, which is visualized using a fluorescence microscope.
04:17
A Technique to Isolate Primary Mast Cells from a Murine Peritoneal Cavity
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2025
This video demonstrates a peritoneal lavage method for isolating mature connective tissue-type mast cells from the peritoneal cavity of adult mice. Upon isolating a mixture of primary immune cells from the peritoneal cavity, selective proliferation of mast cells is performed under appropriate culture conditions.
FAQs
Mast cell mediators influence several steps needed for vascular growth. Vascular endothelial growth factor can support endothelial-cell migration and proliferation, while histamine increases vascular permeability. Proteases contribute to extracellular-matrix remodeling, helping alter the tissue environment through which endothelial cells move. Together, these effects connect mast cell activation with the cellular and structural changes required for new-vessel development.
Degranulation provides a rapid mechanism for releasing stored or available mast cell mediators into surrounding tissue. This release can change vascular permeability and promote conditions favorable to endothelial-cell activity. Its importance lies in linking an inflammatory activation event to vascular remodeling, so the extent and timing of mast cell activation may influence how strongly angiogenic responses develop.
These mediators affect complementary aspects of the angiogenic environment. Histamine can increase vascular permeability, vascular endothelial growth factor supports endothelial-cell migration and proliferation, and proteases help remodel the extracellular matrix. Considering them separately clarifies why mast cell signaling can influence both vessel-associated cells and the surrounding tissue rather than acting through a single biological effect.
The process is particularly relevant in tissues undergoing inflammation, repair, or disease. In these settings, mast cell activation can connect immune signaling with vascular growth, potentially altering the local tissue environment. This context helps explain why the same cellular mechanism is studied across different biological situations, including normal regenerative responses and pathological processes associated with persistent inflammation.
Researchers can examine how mast cell activation and mediator release relate to endothelial-cell migration, endothelial-cell proliferation, vascular permeability, and extracellular-matrix remodeling. Comparing these outcomes across inflammatory, repair, or disease-related tissue contexts can reveal which effects accompany angiogenic activity. Such observations also help identify signaling pathways that may be relevant for controlling abnormal blood-vessel formation.
Mast cell-mediated angiogenesis is studied in tumors and chronic inflammatory disorders because immune-derived signals may support blood-vessel growth in disease-associated tissues. In tumors, this connection is relevant to vascularization, while chronic inflammation provides a setting for sustained immune and vascular interactions. Studying these links can help researchers identify pathways that might be targeted to limit abnormal angiogenesis.