Human Cd31 Antibody
Human Cd31 Antibody - Related Videos
05:32
Purification of CD31+ Endothelial Precursor Cells Using Magnetic-Activated Cell Sorting
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2025
This video demonstrates a technique for purifying CD31+ endothelial progenitor cells (EPCs) from a culture using magnetic-activated cell sorting (MACS). The cell suspension is treated with a fluorophore-conjugated antibody that binds to CD31 on the EPCs. Completing the labeling of CD31+ EPCs involves adding an antibody complex, which binds to the fluorophores, and a polymer-coated magnetic nanoparticle, which binds to the antibody complex. The labeled cells are then purified using a magnetic...
03:18
Antibody-Dependent Enhancement of Zika Virus by Dengue Antibodies in Human Primary Macrophages
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2026
Source: Asad, S., et al. Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells. J. Vis. Exp. (2019)This video demonstrates antibody-dependent enhancement of Zika virus infection in human primary macrophages, using dengue antibody–virus complexes to model how cross-reactive antibodies facilitate Fcγ receptor–mediated viral entry and replication for studying infection mechanisms and immune interactions.
05:33
A Microneutralization Assay to Measure Neutralizing Antibody Titers in Human Serum
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2025
This video demonstrates Microneutralization assays to measure neutralizing antibodies using Madin-Darby Canine Kidney cells overexpressing α2,6-linked sialic acid in human sera. This assay is often used for influenza human...
08:17
A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
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Cited by 9 •
2016
Whole blood Immunophenotyping is indispensable for monitoring the human immune system. However, the number of reagents required and the instability of specific fluorochromes in premixed cocktails necessitates daily reagent preparation. Here we show that semi-automated preparation of staining antibody cocktail helps establish reliable immunophenotyping by minimizing variability in reagent dispensing.
10:32
Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
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Cited by 7 •
2015
Synthesis of human monoclonal antibodies is the first step in studies aimed at unraveling the pathophysiological mechanisms of auto-antibody-mediated immune responses. We have developed a protocol to generate recombinant human immunoglobulin G (IgG) monoclonal antibodies from blood sorted B cells, including B-cell isolation, antibody cloning and in vitro synthesis.
FAQs
The antibody binds a specific epitope, or molecular recognition site, on the CD31 protein. Detection then depends on the labeling format: the antibody may carry a conjugated signal directly, or a labeled secondary antibody may bind to it. This binding-and-detection arrangement converts CD31 presence into an observable readout for analyzing cells or tissue.
CD31 is prominent on endothelial cells but is also expressed by selected blood cells. Consequently, positive staining can support identification and characterization of endothelial populations without automatically proving that every labeled cell is endothelial. Interpreting the signal within the sample’s cellular and tissue context is important when studying vascular structures or related biology.
Specificity, sample preparation, and validated experimental conditions are central determinants of performance. Specificity affects whether the observed signal reflects CD31 recognition, while preparation can influence how well the target remains detectable. Using conditions validated for the chosen assay helps produce interpretable staining and reduces uncertainty when comparing endothelial or vascular samples.
The same target-recognition principle can support different types of analysis. Flow cytometry is suited to detecting and characterizing labeled cell populations, whereas immunohistochemistry and immunofluorescence preserve a tissue-oriented view of staining. These formats therefore provide complementary information, ranging from population-level CD31 detection to the localization of vascular structures within tissue.
A typical workflow begins with preparing the sample under conditions appropriate for the selected assay, followed by exposure to the antibody so it can recognize its CD31 epitope. Detection then uses either the antibody’s conjugated signal or a labeled secondary antibody. The resulting readout is interpreted according to the assay format and the sample’s biological context.
CD31 staining can help researchers characterize endothelial populations and assess vascular structures in tissue. In broader biology studies, the resulting information supports investigations of cell adhesion, leukocyte migration, angiogenesis, and vascular development. These applications connect molecular detection of CD31 with changes in vascular organization and the behavior of cells associated with blood vessels.