Microwell Array

Microwell arrays are patterned substrates containing many small, regularly spaced wells that compartmentalize cells, particles, or small-volume biological samples for parallel analysis. Each well acts as a defined microenvironment: cells or other materials settle into individual recesses, where physical confinement supports controlled cell positioning, interaction, and growth while limiting mixing between compartments. In biology, microwell arrays enable high-throughput single-cell studies, clonal culture, spheroid and organoid formation, and screening assays, often improving sample organization and measurement consistency. Their scalable format supports investigations of cell behavior, development, disease models, and responses to experimental treatments.

Microwell Array - Related Videos

Research

JoVE EoE - Bacterial Growth and Techniques

Photodegradable Hydrogel-Based Isolation of Bacterial Colonies in Microwell Arrays

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2025

Source: Fattahi, N., et al. Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation. J. Vis. Exp. (2021)This video demonstrates the use of photodegradable hydrogels to isolate bacterial colonies within silicon microwell arrays. Bacterial cells are first loaded into microwells, encapsulated in a crosslinked hydrogel, and cultured into distinct colonies. Upon exposure to ultraviolet light, targeted degradation of the hydrogel enables selective extraction of individual...

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform

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Cited by 12 •

2017

The development of microbial communities depends on a combination of factors, including environmental architecture, member abundance, traits, and interactions. This protocol describes a synthetic, microfabricated environment for the simultaneous tracking of thousands of communities contained in femtoliter wells, where key factors such as niche size and confinement can be approximated.

A Paired Bead and Magnet Array for Molding Microwells with Variable Concave Geometries

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Cited by 8 •

2018

This manuscript introduces a robust method of fabricating concave microwells without the need for complex high-cost facilities. Using magnetic force, steel beads, and a through-hole array, several hundred microwells were formed in a 3 cm x 3 cm polydimethylsiloxane (PDMS) substrate.

Research

JoVE Journal - Bioengineering
Free Sample

Production of Large Numbers of Size-controlled Tumor Spheroids Using Microwell Plates

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Cited by 36 •

2013

We introduce a protocol for the generation of large numbers (thousands to hundreds of thousands) of uniform size- and composition-controlled tumor spheroids, using commercially available microwell plates.

Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells

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Cited by 11 •

2016

Conventional methods to initiate suspension aggregate based cardiac differentiation of human pluripotent stems cells (hPSCs) are plagued with culture heterogeneity with respect to aggregate size and shape. Here, we describe a robust method for cardiac differentiation employing microwells to generate size-controlled hPSC aggregates cultured under cardiac-promoting conditions.

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