Mortality Bioassay

A mortality bioassay is a biological test that measures the death or survival of organisms after exposure to a substance, environmental condition, or treatment, providing a standardized way to assess harmful effects. In a controlled experiment, organisms are assigned to treated and control groups, exposed for a defined period, and evaluated using mortality as the endpoint across different concentrations or conditions. Researchers use the resulting concentration-response patterns to estimate toxicity and compare the effects of chemicals, pesticides, pollutants, or biological agents. Mortality bioassays support environmental monitoring, risk assessment, pharmacological research, and the study of organismal responses to stress.

Mortality Bioassay - Related Videos

Research

JoVE Journal - Biology
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Bioassays for Monitoring Insecticide Resistance

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Cited by 45 •

2010

This manuscript demonstrates and discusses techniques used to survey pesticide susceptibility and detect resistance to contact and systemic pesticides in arthropod pests.

Research

JoVE Journal - Biology

Bone Conditioned Medium: Preparation and Bioassay

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Cited by 13 •

2015

We describe here how to prepare bone-conditioned medium (BCM) and test its activity in vitro.

Assessing Pathogenic Bacteria–Induced Mortality in C. elegans Larvae

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2025

Source: Anderson, Q. L., et al. A High-throughput, High-content, Liquid-based C. elegans Pathosystem. J. Vis. Exp. (2018)The video demonstrates a liquid-based assay where genetically modified C. elegans larvae are exposed to pathogenic bacteria in a microplate. Bacterial toxins secreted into the medium induce host stress and death. A fluorescent dye stains dead worms, allowing imaging-based quantification of mortality to assess genetic susceptibility or resistance.

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

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Cited by 7 •

2016

We describe a simple cell-based bioassay for detecting, quantifying and monitoring the activity of members of the vascular endothelial growth factor family of ligands. The assay uses chimeric receptors expressed in a factor-dependent cell line to provide a semi-quantitative or quantitative assessment of receptor binding and cross-linking by the ligand.

Electroantennographic Bioassay as a Screening Tool for Host Plant Volatiles

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Cited by 14 •

2012

A method to rapidly screen host plant volatiles by measurement of the electrophysiological response of adult navel orangeworm (Amyelois transitella) antennae to single components and blends via electroantennographic analysis is demonstrated.

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