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Bone Conditioned Medium: Preparation and Bioassay
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JoVE Journal Biology
Bone Conditioned Medium: Preparation and Bioassay

Bone Conditioned Medium: Preparation and Bioassay

DOI:
10.3791/52707-v

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07:18 min

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July 08, 2015

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, , , , ,
1Department of Oral Surgery and Stomatology,School of Dental Medicine, University of Bern, 2Laboratory of Oral Cell Biology,School of Dental Medicine, University of Bern, 3Department of Oral and Maxillofacial Surgery,School of Dental Medicine, Universitat Internacional de Catalunya, 4Robert K. Schenk Laboratory of Oral Histology,School of Dental Medicine, University of Bern, 5Department of Cranio Maxillofacial Surgery,Inselspital, University of Bern, 6Department of Implant Dentistry,School of Dentistry, Universidade Federal de Santa Catarina

Chapters

  • 00:05Title
  • 01:51Preparation of Bone Conditioned Medium
  • 03:44Mesenchymal Cell Based Bioassays
  • 05:30Results: Bone Conditioned Medium Promotes Osteoinductive Gene Expression
  • 06:21Conclusion

Summary

Automatic Translation

Automatic Translation

We describe here how to prepare bone-conditioned medium (BCM) and test its activity in vitro.

Tags

Keywords: Bone-conditioned MediumAutologous Bone GraftsOsteoconductivityOsteogenicityOsteoinductivityMesenchymal CellsTGF-βIn Vitro BioassayReconstructive Surgery

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