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Bone Conditioned Medium: Preparation and Bioassay

Bone Conditioned Medium: Preparation and Bioassay

JoVE Journal
Biology

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JoVE Journal Biology

Bone Conditioned Medium: Preparation and Bioassay

Bone Conditioned Medium: Preparation and Bioassay

11,014 Views

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07:18 min

•

July 08, 2015

DOI:

10.3791/52707-v

DOI:

10.3791/52707-v

•

07:18 min

July 08, 2015

•

10 Views

Jordi Caballé-Serrano^1,2,3, Kosaku Sawada^2,5, Guenther Schuldt Filho^1,2,6, Dieter D. Bosshardt^1,4, Daniel Buser¹, Reinhard Gruber^1,2

¹Department of Oral Surgery and Stomatology,School of Dental Medicine, University of Bern, ²Laboratory of Oral Cell Biology,School of Dental Medicine, University of Bern, ³Department of Oral and Maxillofacial Surgery,School of Dental Medicine, Universitat Internacional de Catalunya, ⁴Robert K. Schenk Laboratory of Oral Histology,School of Dental Medicine, University of Bern, ⁵Department of Cranio Maxillofacial Surgery,Inselspital, University of Bern, ⁶Department of Implant Dentistry,School of Dentistry, Universidade Federal de Santa Catarina

Summary

We describe here how to prepare bone-conditioned medium (BCM) and test its activity in vitro.

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