Neuroendocrine System
Neuroendocrine System - Related Videos
12:04
Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy
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Cited by 39 •
2012
We present a simple agarose overlay platform to grow 3D multicellular spheroids using neuroendocrine cancer cell lines. This method provides a very convenient way to examine the effect of therapeutic drugs on the neuroendocrine tumor cells. It could also help us establish human neuroendocrine tumor spheroids for cancer therapy.
02:38
Characterization of Small Bowel Neuroendocrine Tumor Spheroids Using Immunofluorescence
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2025
Source: Ear, P. H., et. al. Establishment and Characterization of Small Bowel Neuroendocrine Tumor Spheroids. J. Vis. Exp. (2019)This video demonstrates the immunofluorescence staining of small bowel neuroendocrine tumor spheroids. The spheroids are fixed, permeabilized, and blocked and are then incubated with primary and secondary antibodies. A dye is added to stain nuclei before mounting and visualizing them under a fluorescent microscope.
09:43
Establishment and Characterization of Small Bowel Neuroendocrine Tumor Spheroids
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Cited by 19 •
2019
Neuroendocrine tumors (NETs) originate from neuroendocrine cells of the neural crest. They are slow growing and challenging to culture. We present an alternative strategy to grow NETs from the small bowel by culturing them as spheroids. These spheroids have small bowel NET markers and can be used for drug testing.
11:50
Genome-Wide Mapping of Histone Modifications and Transcription Factor Binding Sites in Neuroendocrine Small Cell Lung Cancer Cell Lines Using CUT&RUN
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2026
An optimized Cleavage Under Targets and Release Using Nuclease followed by next generation sequencing (CUT&RUN-seq) protocol is described for neuroendocrine small cell lung cancer cell lines. It enables genome-wide mapping of various histone modifications and transcription factor (e.g. E2F7) binding sites to investigate epigenetic and transcriptional deregulation in SCLC pathobiology.
03:20
Splitting Spheroids for Sub-culturing and Shipping: A Procedure for Propagating and Transferring Spheroids from Small Bowel Neuroendocrine Tumor
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2023
This video demonstrates a strategy to split and propagate spheroids developed from small bowel neuroendocrine tumor cells. These spheroids have small bowel neuroendocrine tumor markers and can be propagated and further used to test anti-cancer drugs.
FAQs
The hypothalamus provides a central connection between neural control and hormone regulation, while the pituitary gland and peripheral endocrine organs carry out downstream endocrine functions. Their coordinated activity allows signals originating in the nervous system to influence body-wide processes. Studying these components together helps explain how regulation extends from central control to metabolism, growth, reproduction, and other physiological functions.
Feedback loops adjust hormone secretion as internal conditions change. This regulation prevents signaling from remaining excessively high or low and links endocrine activity to variables such as stress, energy availability, growth, and reproduction. In biological research, examining these loops helps identify how disrupted regulation may contribute to hormonal imbalance and related disorders.
Neural signaling can provide rapid coordination, whereas hormonal regulation produces effects that last longer throughout the body. The neuroendocrine system connects these different timescales through specialized neurons that release hormones or control endocrine glands. This arrangement supports both immediate responses and sustained regulation of functions such as stress responses, metabolism, development, and circadian rhythms.
Neuroendocrine activity responds to changing internal conditions, including stress, energy availability, growth, and reproductive status. These inputs influence secretion through regulatory feedback, allowing body functions to adjust rather than operate independently of physiological state. The resulting coordination is important for maintaining homeostasis and for understanding how changes in internal conditions can affect hormonal balance.
This system is particularly relevant when researchers study hormonal imbalance, stress responses, fertility, growth, or metabolic disease. Its integrated signaling provides a framework for connecting changes in neural regulation with endocrine outcomes. Research may also consider circadian rhythms and immune activity, because both are among the body functions influenced by neuroendocrine coordination.
Research on neuroendocrine signaling can clarify how the body maintains homeostasis while coordinating metabolism, development, circadian rhythms, immune activity, and reproduction. It can also help connect altered regulation with disorders involving stress, growth, fertility, hormonal imbalance, or metabolism. This broad scope makes the topic useful for linking cellular signaling principles with organism-level biological outcomes.