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Establishment and Characterization of Small Bowel Neuroendocrine Tumor Spheroids
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Chapters
- 00:04Title
- 00:44Small Bowel Neuroendocrine Tumor (SBNET) Collection and Cell Dissociation
- 02:10Culture of SBNET as Tumor Spheroids in ECM
- 04:02Characterization of SBNET Spheroids by Immunofluorescence
- 05:50Splitting SBNET Spheroids
- 07:23Results: Immunofluorescence Imaging and Drug Testing of SBNET Spheroids
- 09:02Conclusion
Neuroendocrine tumors (NETs) originate from neuroendocrine cells of the neural crest. They are slow growing and challenging to culture. We present an alternative strategy to grow NETs from the small bowel by culturing them as spheroids. These spheroids have small bowel NET markers and can be used for drug testing.
