Pierceable Sealing Film
Pierceable Sealing Film - Related Videos
09:27
Encapsulation and Permeability Characteristics of Plasma Polymerized Hollow Particles
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Cited by 1 •
2012
We have used plasma enhanced chemical vapor deposition to deposit thin films ranging from a few nm to several 100 nm on nano-sized particles of various materials. We subsequently etch the core material to produce hollow nanoshells whose permeability is controlled by the thickness of the shell. We characterize the permeability of these coatings to small solutes and demonstrate that these barriers can provide sustained release of the core material over several days.
07:58
Electroplating of Thin Films
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2023
Source: Logan G. Kiefer, Andrew R. Falkowski, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Electroplating is a process that uses electric current to reduce dissolved metal cations so that they form a thin coating on an electrode. Other thin film deposition techniques include chemical vapor deposition (CVD), spin coating, dip coating, and sputter deposition among others. CVD uses a gas-phase precursor of the element to be...
04:41
Optimized Sealing Process and Real-Time Monitoring of Glass-to-Metal Seal Structures
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Cited by 7 •
2019
Key procedures to optimize the sealing process and achieve real-time monitoring of the metal-to-glass seal (MTGS) structure are described in detail. The embedded fiber Bragg grating (FBG) sensor is designed to achieve online monitoring of temperature and high-level residual stress in the MTGS with simultaneous environmental pressure monitoring.
06:37
An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium
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Cited by 11 •
2012
We describe a live whole animal quantitative measurement for permeability of the embryonic zebrafish brain. The technique analyzes the ability to retain cerebrospinal fluid and molecules of different molecular weights within the neural tube lumen and quantifies their movement out of the ventricles. This method is useful for determining differences in epithelial permeability and maturation during development and disease.
10:08
Fabrication of Large-area Free-standing Ultrathin Polymer Films
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Cited by 10 •
2015
We describe a method for the fabrication of large-area (up to 13 cm diameter) and ultrathin (as thin as 8 nm) polymer films. Instead of using a sacrificial interlayer to delaminate the film from its substrate, we use a self-limiting surface treatment suitable for arbitrarily large areas.
FAQs
Its thin, compliant material creates a protective barrier over the container or plate, reducing exposure of samples to the surrounding environment. The barrier also helps limit evaporation and sample loss during incubation or storage steps. Because researchers can access selected locations rather than opening the entire vessel, neighboring wells or samples remain less exposed during handling.
Flexibility allows the film to conform to the covered surface while remaining thin enough for penetration by pipette tips or needles. This combination supports closure without requiring complete removal during access. In biological experiments, maintaining coverage while selectively reaching samples helps preserve containment and reduces unnecessary handling across a plate or vessel.
Selective piercing reduces the number of handling steps needed to reach individual samples. It also avoids exposing all wells or container contents at once, which can help protect neighboring samples from contamination, evaporation, or accidental loss. This approach is particularly useful when a workflow requires repeated or location-specific access within a covered microplate.
Researchers place the film over a microplate, culture vessel, or sample container, then penetrate the covering at the required location with a pipette tip or needle. Access occurs through selected points while the remaining covered areas stay protected. The method supports controlled addition, removal, or handling of biological samples without taking away the entire covering.
The material supports several workflows identified in biological research, including cell culture, reagent incubation, nucleic acid preparation, and high-throughput assays. In each case, the covering helps balance sample protection with practical access. Its use is most relevant when researchers need to handle selected wells or samples while limiting exposure of the rest of the experiment.
By reducing full-seal removal and unnecessary handling, the film can make access to selected wells more consistent across a workflow. Limiting exposure of neighboring wells also helps preserve sample integrity during processing. These effects support more uniform handling conditions, which can contribute to improved workflow consistency and reproducible biological experiments.