Plant Expression System
Plant Expression System - Related Videos
11:26
Robotics and Dynamic Image Analysis for Studies of Gene Expression in Plant Tissues
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Cited by 18 •
2010
We report a method for introduction, tracking and quantitative analysis of GFP expression in plant cells. This method utilizes a custom-designed robotics system for semi-continuous image collection from large numbers of samples, over time. We also demonstrate the use of ImageJ and ImageReady for analysis of image series.
13:05
Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
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Cited by 9 •
2014
Tobacco plants were used to produce a fungal cellulase, TrCel5A, via a transient expression system. The expression could be monitored using a fluorescent fusion protein, and the protein activity was characterized post-expression.
07:50
Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
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Cited by 200 •
2013
Plants offer a novel system for the production of pharmaceutical proteins on a commercial scale that is more scalable, cost-efficient and safe than current expression paradigms. In this study, we report a simple and convenient, yet scalable approach to introduce target-gene containing Agrobacterium tumefaciens into plants for protein transient expression.
02:36
Single-Cell Analysis of Gene Expression from Plant-Extracted Pathogens
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2026
Source: Rufián, J. S., et al. Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022).This video demonstrates a method for analyzing gene expression in plant-extracted pathogenic bacteria at the single-cell level using a fluorescently tagged transcription factor. Confocal imaging reveals heterogeneity in virulence gene expression, highlighting functional diversity within a clonal bacterial population.
11:04
Geomagnetic Field (Gmf) and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression
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Cited by 31 •
2015
This protocol describes a method to test the hypothesis that the reversal of the effective geomagnetic field (GMF) induces differential gene expression and alters the morphology of Arabidopsis thaliana. A triaxial octagonal system of Helmholtz coil-pairs was used to artificially generate reversed GMF conditions in the plant growth chamber.
FAQs
In a Plant Expression System, regulatory sequences determine whether the introduced gene is transcribed into messenger RNA. That RNA then serves as the template for translation, the cellular process that produces the recombinant protein. Because these control elements connect gene introduction with protein production, they are central to obtaining the intended molecular product.
Transient expression provides rapid, short-term production, whereas stable expression is designed for persistence. In the stable format, the transgene integrates into the plant genome and can pass to subsequent generations. The transient format is therefore suited to limited-duration production, while stable expression supports continued inheritance across plant generations.
Two commonly used routes for introducing a gene of interest are Agrobacterium-mediated transformation and particle bombardment. Both are presented as ways to deliver genetic information into plant cells, after which regulatory sequences can drive transcription. Their shared purpose is to establish the molecular starting point for producing the desired recombinant protein.
After gene delivery, the production pathway proceeds through transcription, messenger RNA formation, and translation. Transcription copies information from the introduced gene into messenger RNA, and translation uses that RNA to produce the target protein. Following this sequence connects the initial genetic modification with the recombinant product generated by the plant cells or tissues.
Plant Expression Systems can be selected when the target product is a vaccine, antibody, enzyme, or another protein of interest. This range makes the platform relevant to molecular biology, agriculture, and pharmaceutical development. The specific product determines the practical research goal, whether scientists are producing a protein or studying it in a biological setting.
Scalability is a major reason these systems matter beyond individual experiments. The platform supports scalable approaches relevant to molecular biology, agriculture, and pharmaceutical development. Consequently, a Plant Expression System can connect basic studies of gene expression and recombinant proteins with broader efforts to develop useful protein products in applied research.