Sporulation

Sporulation is the biological process by which organisms produce spores, specialized cells that support reproduction, dispersal, or survival under unfavorable conditions. In bacteria such as Bacillus, nutrient limitation triggers asymmetric cell division, chromosome segregation, and formation of a resistant endospore within the mother cell; in fungi and plants, sporulation can involve mitosis or meiosis to generate genetically similar or varied spores. This process helps organisms persist through environmental stress and spread to new habitats. Studying sporulation is important in microbiology, agriculture, ecology, and biotechnology, including the control of pathogenic bacterial spores and the use of fungal spores in research and crop production.

Sporulation - Related Videos

Research

JoVE Journal - Biology

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format

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Cited by 3 •

2016

Here, sporulation of Saccharomyces cerevisiae is carried out in a 96 multiwell format.

Education

JoVE Core - Microbiology

Endospores and Sporulation

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2025

Endospores are specialized, dormant cells primarily formed by Gram-positive bacteria, including Bacillus and Clostridium, enabling survival under extreme environmental conditions. Due to their unique composition and formation process, these structures are highly resistant to physical and chemical insults, such as extreme heat, ultraviolet and ionizing radiation, desiccation, and toxic chemicals. Rare instances of endospore-like structures have also been observed in some Gram-negative bacteria,...

Gene Regulation During Sporulation

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2025

Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...

Research

JoVE Journal - Biology
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BEST: Barcode Enabled Sequencing of Tetrads

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Cited by 4 •

2014

Barcode Enabled Sequencing of Tetrads (BEST) replaces the manual processes of isolating, disrupting and spacing tetrads. BEST isolates tetrads by fluorescence-activated cell sorting onto agar plates, separates the spores by agitation with glass beads, and determines which randomly arrayed colonies were derived from the same original tetrad using molecular barcodes.

Production and Aerosol-Based Deposition of Bacillus subtilis Spores

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2025

Source: Fuchs, F. M., et al. Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy. J. Vis. Exp. (2017)This video demonstrates the production, purification, and aerosol-based deposition of Bacillus subtilis spores to create a uniform monolayer, enabling downstream applications such as microscopy-based analysis of spore germination, viability, or stress response.

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