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Chapters
- 00:05Title
- 01:54Sporulating Library of Transformed Cells to Obtain Barcoded Tetrads
- 03:29Establishing the FACS Gates for Tetrad Sorting
- 06:14Sorting Tetrads Onto an Agar Plate
- 08:16Separating Spores
- 09:25Harvesting Colonies
- 10:54Results: BEST is an Efficient Way to Isolate Spores from Tetrads
- 12:37Conclusion
Barcode Enabled Sequencing of Tetrads (BEST) replaces the manual processes of isolating, disrupting and spacing tetrads. BEST isolates tetrads by fluorescence-activated cell sorting onto agar plates, separates the spores by agitation with glass beads, and determines which randomly arrayed colonies were derived from the same original tetrad using molecular barcodes.
