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JoVE Journal

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BEST: Barcode Enabled Sequencing of Tetrads

Article DOI: 10.3791/51401
May 1st, 2014


Summary May 1st, 2014

Barcode Enabled Sequencing of Tetrads (BEST) replaces the manual processes of isolating, disrupting and spacing tetrads. BEST isolates tetrads by fluorescence-activated cell sorting onto agar plates, separates the spores by agitation with glass beads, and determines which randomly arrayed colonies were derived from the same original tetrad using molecular barcodes.

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