Surgical Amputation
Surgical Amputation - Related Videos
06:49
Rat Hindlimb Below-Knee Amputation Model and Immediate Targeted Muscle Reinnervation
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2026
This protocol demonstrates how to perform a below-knee amputation in rats and to immediately perform targeted muscle reinnervation (iTMR) to investigate strategies to treat and prevent amputation-related pain and to better understand pain mechanisms associated with amputation.
03:58
Apical Resection Neonatal Mouse Model: A Surgical Procedure to Amputate the Ventricular Apex to Study Regenerative Potential in a Neonate Mouse Heart
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2025
In this video, we demonstrate the procedure to surgically remove the left ventricular apex of a neonatal mouse heart.
06:14
Chemical Amputation and Regeneration of the Pharynx in the Planarian Schmidtea mediterranea
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Cited by 3 •
2018
The planarian Schmidtea mediterranea is an excellent model for studying stem cells and tissue regeneration. This publication describes a method to selectively remove one organ, the pharynx, by exposing animals to the chemical sodium azide. This protocol also outlines methods for monitoring pharynx regeneration.
07:28
Microscopic Replantation of Penile Glans Amputation Due to Circumcision
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Cited by 2 •
2022
The present protocol describes the emergency management of microscopic replantation of penile glans amputation due to circumcision.
01:32
Pig Surgical Preparation and Postoperative Care
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Surgical Preparation and Physiological Monitoring This video provides an introductory overview of surgical preparation, intraoperative monitoring, and postoperative care in swine. It is intended to familiarize learners with key...
FAQs
The remaining tissue undergoes inflammation, followed by wound healing and scar formation. These responses help close and stabilize the surgical site while the residual limb develops its lasting tissue structure. At the same time, sensory and motor signaling can change, making the procedure relevant not only to tissue repair but also to later rehabilitation and pain management.
Careful shaping creates a stable residual limb rather than leaving an irregular or poorly supported tissue structure. Preserving healthy tissue supports healing, while controlling bleeding and arranging bone and muscle helps the remaining limb close effectively. These biological and surgical considerations influence how the residual limb functions during rehabilitation and how it can support prosthetic design.
Removing a body part alters the biological pathways associated with sensation and movement. The nervous system must respond to these changes while the residual limb heals, producing a context for neural adaptation, or adjustment in signaling over time. Studying this adaptation helps connect surgical outcomes with rehabilitation strategies and approaches to pain management.
The central consideration is whether tissue remains viable enough to preserve. Severe injury, disease, infection, or tissue death can make preservation unsuitable, whereas healthy structures are retained when possible. This balance determines the condition of the residual limb, supports wound healing, and affects the biological foundation for later rehabilitation and prosthetic use.
Researchers can follow the transition from inflammation to wound healing and scar formation, then examine how the residual limb and its signaling systems adapt. These stages provide different kinds of information: early tissue response, closure and structural stabilization, and longer-term sensory or motor changes. Together, they help evaluate healing and guide rehabilitation research.
The procedure provides a context for studying how residual tissues heal, how sensory and motor signals change, and how the body adapts after tissue loss. Findings can support prosthetic design and rehabilitation, while also informing research on pain management, neural adaptation, and tissue regeneration. Each application addresses a different biological consequence of the surgical change.