Syringe Mixer Method
Syringe Mixer Method - Related Videos
12:42
Microfluidic Mixers for Studying Protein Folding
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Cited by 9 •
2012
In this work we explain the fabrication and use of a microfluidic mixer capable of mixing two solutions in ~8 μs. We also demonstrate the use of these mixers with spectroscopic detection using UV fluorescence and fluorescence resonance energy transfer (FRET).
02:28
Extraction of Apoplastic Bacteria Using a Syringe-Based Pressure Method
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2025
Source: Rufián, J. S., et al, Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a syringe-based pressure method for extracting apoplastic bacteria, which proliferate and form microcolonies within the apoplast, the intercellular space between plant cells. The method yields debris-free bacterial samples suitable for downstream single-cell analysis.
08:10
Synthesizing Lipid Nanoparticles by Turbulent Flow in Confined Impinging Jet Mixers
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Cited by 20 •
2024
A detailed protocol for synthesizing lipid nanoparticles (LNPs) using confined impinging jet (CIJ) mixer technologies, including a two-jet CIJ and a four-jet multi-inlet vortex mixer (µMIVM), is demonstrated. The CIJ mixers generate reproducible, turbulent micro-mixing environments, resulting in the production of monodisperse LNPs.
09:58
Syringe-injectable Mesh Electronics for Stable Chronic Rodent Electrophysiology
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Cited by 31 •
2018
Mesh electronics probes seamlessly integrate and provide stable, long-term, single-neuron level recording within the brain. This protocol uses mesh electronics for in vivo experiments, involving the fabrication of mesh electronics, loading into needles, stereotaxic injection, input/output interfacing, recording experiments, and histology of tissue containing mesh probes.
05:49
Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle
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Cited by 2 •
2021
Here we demonstrate a method to apply fluid shear stress to cancer cells in suspension to model the effects of hemodynamic stress on circulating tumor cells.
FAQs
Repeatedly moving liquid between connected syringes transports material through the bulk fluid while the narrow connector or passage generates shear. Advection helps carry solutes, cells, particles, or polymers across the sample, and shear promotes their redistribution. Together, these effects improve compositional uniformity without relying on a conventional stirring vessel.
Syringe volume, transfer rate, and the number of transfer passes are the main variables identified for controlling the outcome. Changing the volume alters how much material is handled at once, while transfer rate affects sample movement through the connector. Additional passes can further redistribute components, although sample handling conditions remain important.
The approach is suited to small-volume preparations and uses connected syringes rather than a conventional stirring setup. This provides a controlled way to move a sample repeatedly through a narrow passage, which can be practical when the available volume or formulation makes ordinary stirring unsuitable. It also supports reproducible preparation across repeated workflows.
Its mixing action can distribute several types of material, including dissolved solutes, cells, particles, and polymers. The relevant outcome is not limited to a simple solution: repeated syringe transfers can help produce a more uniform suspension or formulation. Because sample handling influences the result, the transfer settings should match the material being prepared.
A basic workflow uses two connected syringes and a connector or narrow passage. The biological or chemical liquids are placed in the syringe system, then transferred back and forth repeatedly. The operator controls syringe volume and transfer rate and selects the number of passes needed to obtain the desired degree of distribution.
Biology workflows may use it for reagent preparation, sample homogenization, and creating suspensions or other mixed formulations. It is especially relevant when researchers need controlled handling of small volumes or must combine components that conventional stirring does not readily accommodate. The method can also fit both teaching laboratories and specialized experimental workflows.
The principal outcome is a more uniformly distributed mixture, whether the sample contains solutes, cells, particles, or polymers. Consistent control of syringe volume, transfer rate, and pass number can support reproducible preparation. The resulting material may serve as a reagent, homogenized biological sample, suspension, or other formulation for downstream work.
Its simple syringe-based setup makes the underlying effects of fluid movement, advection, and shear accessible in teaching laboratories. At the same time, controlled transfer conditions can support specialized workflows that require small-volume reagent preparation, homogenization, or formulation. This combination of simplicity and controllability extends its relevance across different biological laboratory settings.