T Lymphocyte Lines
T Lymphocyte Lines - Related Videos
08:39
Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
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Cited by 27 •
2010
T lymphocyte migration occurs during homing to lymphoid organs, exit from the vasculature, and entering into peripheral tissues. Here, we describe a protocol that can be used to analyze T lymphocyte migration in vitro.
08:08
Isolation and Activation of Murine Lymphocytes
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Cited by 56 •
2016
Lymphocytes are the major players in adaptive immune responses. Here, we present a lymphocyte purification protocol to determine the physiological functions of the desired molecules in lymphocyte activation in vitro and in vivo. The described experimental procedures are suitable for comparing functional capacities between control and genetically modified lymphocytes.
12:59
Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
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Cited by 33 •
2013
With effector functions distinct from other T cell subsets, Th17 cells have been centrally implicated in inflammatory autoimmunity. This in vitro Th17 differentiation protocol provides a means to determine whether naïve CD4+ T lymphocytes can differentiate into Th17 cells, and to further examine their role in autoimmunity and host response.
06:02
Isolation of Lymphocytes from a Mouse Peyer's Patch
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2025
In this video, we demonstrate the isolation of lymphocytes from a harvested mouse Peyer's patch. The isolated lymphocytes can be used for further analysis.
08:09
Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils
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Cited by 11 •
2015
Palatine tonsils are a rich source of B and T lymphocytes. Here we provide an easy, efficient and rapid protocol to isolate B and T lymphocytes from human palatine tonsils. The method described has been specifically adapted for studies of the viral etiology of tonsil inflammation known as tonsillitis.
FAQs
Antigen recognition initiates signaling through the T-cell receptor and its associated CD3 complex. This receptor machinery links an external activation event to intracellular changes that can alter proliferation and cellular function. Studying this response in a controlled population helps researchers examine how adaptive immune cells convert receptor engagement into measurable biological outcomes.
Cytokines and other activation signals influence both the expansion of the cells and the functional changes they display. Their effects can therefore shape experimental outcomes even when the receptor stimulus remains constant. Researchers vary or monitor these signals to investigate how environmental cues regulate T-cell behavior, rather than attributing every response solely to antigen-receptor engagement.
Laboratory lines provide reproducible and renewable experimental material, but they do not represent every feature of primary T cells or whole organisms. Results may therefore identify signaling, gene-expression, or functional patterns that require broader confirmation. Comparing model-line findings with primary-cell and in vivo evidence strengthens interpretation and helps distinguish general biology from model-specific behavior.
Researchers maintain the cells under controlled culture conditions, expose them to selected antigen-receptor or activation signals, and then measure responses such as proliferation, functional changes, or gene expression. The same population can support repeated experiments because it is renewable and consistent. This workflow enables controlled comparisons of signaling conditions, treatments, or cellular interactions.
Depending on the experimental design, these lines can provide information about T-cell development, signaling pathways, gene expression, cytotoxicity, and interactions with infected or transformed cells. Such readouts connect receptor stimulation to cellular behavior and help investigators compare how different conditions influence immune functions. The resulting data are especially useful for dissecting mechanisms in a controlled setting.
Their reproducibility makes them useful for studying adaptive immunity and lymphocyte biology across immunology, virology, cancer biology, and drug development. Investigators can examine responses to infected or transformed cells, analyze signaling or cytotoxicity, and test how treatments affect T-cell behavior. Findings can guide follow-up studies while still requiring comparison with primary cells or in vivo systems.