Tissue Removal
Tissue Removal - Related Videos
02:43
Deparaffinization and Rehydration of Tissue Sections: A Two-step Procedure to Remove Paraffin Wax from FFPE Tissue Slides Followed by Tissue Rehydration
0 Views •
2023
In this video, we demonstrate the processing of FFPE or formalin-fixed paraffin-embedded sections obtained from gastrointestinal tumor tissue. The deparaffinization and rehydration steps allow the processing of the tissue specimen for subsequent analytical applications.
04:37
Sampling Prostate Cancer Tissue for Biobanking: An MRI-guided Targeted Technique to Remove Biopsy Punches from Fresh Tumor Tissue
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2023
This video demonstrates an MRI data-based technique to target specific tumor-containing regions in prostate tissue for sampling. The collected biopsy specimens can be either stored for biobanking purposes or used for subsequent downstream analysis.
06:41
Removal of Drosophila Muscle Tissue from Larval Fillets for Immunofluorescence Analysis of Sensory Neurons and Epidermal Cells
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Cited by 13 •
2016
Studies of neuronal morphogenesis using Drosophila larval dendritic arborization (da) neurons benefit from in situ visualization of neuronal and epidermal proteins by immunofluorescence. We describe a procedure that improves immunofluorescence analysis of da neurons and surrounding epidermal cells by removing muscle tissue from the larval body wall.
06:43
Rotary Evaporation to Remove Solvent
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2023
Source: Dr. Melanie Pribisko Yen and Grace Tang — California Institute of Technology Rotary evaporation is a technique most commonly used in organic chemistry to remove a solvent from a higher-boiling point compound of interest. The rotary evaporator, or "rotovap", was invented in 1950 by the chemist Lyman C. Craig. The primary use of a rotovap is to dry and purify samples for downstream applications. Its speed and ability to handle large volumes of solvent make rotary evaporation a preferred...
03:02
Nictitating Membrane Removal: A Surgical Procedure to Remove Nictitating Membrane from Rabbit Eye
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2025
In this video, we demonstrate a surgical procedure to remove nictitating membrane from rabbit’s eye.
FAQs
These approaches share a central objective: separating tissue by disrupting connections between cells and the extracellular matrix, the material surrounding cells, without unnecessarily losing the sample needed for analysis. Mechanical dissection and surgical excision provide physical separation, whereas chemical or enzymatic treatment can disrupt tissue connections through nonmechanical means. The chosen approach therefore influences how well structural information remains available.
Location and extent are major determinants of interpretation. Removing tissue from a defined site helps connect observed findings to a particular anatomical region, while limiting the amount removed helps distinguish the response to tissue loss from effects caused by a broader injury. Careful control also improves sample quality, making later structural or cellular analysis more reliable.
Tissue loss can produce two overlapping kinds of evidence: findings caused directly by the missing tissue and findings associated with the organism’s wound-healing response. Keeping these possibilities separate is essential when interpreting experiments. A controlled removal site and extent help researchers compare the immediate consequence of tissue loss with subsequent biological responses.
Planning begins with the intended analysis. Researchers select a mechanical, surgical, chemical, or enzymatic approach, identify the tissue location, and determine how much material to remove while preserving what must be examined. These decisions support sample quality and make the resulting observations more interpretable in histology, anatomy, pathology, or regeneration studies.
Its applications span several biological fields. In histology, removed material can be examined for structure; anatomy studies use it to investigate organization; pathology uses samples to evaluate abnormal tissue; regeneration research examines responses after tissue loss. The same controlled preparation can therefore support both descriptive analysis and response-focused experiments.
Separating selected tissue can provide material for isolating specific cell populations, an important use identified in biological research. The value of the preparation depends on controlling the removal site and extent so that the material corresponds to the intended tissue. This supports cellular analysis alongside broader examinations of tissue structure and organization.
Regeneration studies use controlled tissue loss as a way to examine how an organism responds after material has been removed. Researchers can assess the resulting response while considering the original location and extent of loss. This context helps separate regeneration-related observations from general wound-healing effects and links the experiment to the tissue’s structural changes.