Tumor Enrichment
Tumor Enrichment - Related Videos
11:37
In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells
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Cited by 30 •
2015
Tumor-initiating cells (TICs) may represent a viable therapeutic target for the treatment of ovarian cancer, a highly recurrent and fatal disease. We present a protocol for culture conditions that enrich for this highly tumorigenic population of cells.
06:44
Automated Dissection Protocol for Tumor Enrichment in Low Tumor Content Tissues
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Cited by 2 •
2021
Digital annotation with automated tissue dissection provides an innovative approach to enriching tumor in low tumor content cases and is adaptable to both paraffin and frozen tissue types. The described workflow improves accuracy, reproducibility and throughput and could be applied to both research and clinical settings.
09:52
A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication
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Cited by 8 •
2016
We adapted a permeable microporous membrane insert to mimic the tumor microenvironment (TME). The model consists of a mixed cell culture, allows simplified generation of highly enriched individual cell populations without using fluorescent tagging or cell sorting, and permits studying intercellular communication within the TME under normal or stress conditions.
03:46
Stool Microbial DNA Isolation: Studying the Effect of Environmental Enrichment on Microbiome Diversity in Murine Tumor Model
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2023
This video describes DNA isolation protocol from stool samples of colon tumor-bearing mice. The extracted DNA is amplified using PCR, and the product is further cleaned using a magnetic beads-based approach. This method can be used to determine the effect of environmental enrichment on colon microbiota and animal mortality.
08:32
Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
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Cited by 9 •
2018
Here we describe a method to separate and enrich components of the tumor immune and non-immune microenvironment in established subcutaneous tumors. This technique allows for the separate analysis of tumor immune infiltrate and non-immune tumor fractions which can permit comprehensive characterization of the tumor immune microenvironment.
FAQs
Physical selection separates cells according to measurable properties such as size or density, whereas biological selection uses differences in surface-marker expression. These strategies can be applied after tissue dissociation to concentrate malignant cells from a mixed population. The choice of distinguishing feature affects which cells are retained and determines how effectively the sample is enriched for tumor material.
When tumor cells are scarce among stromal, immune, and other normal cells, signals from malignant cells may be diluted by material from the surrounding population. Enrichment increases the relative contribution of tumor-derived material, making genomic, transcriptomic, and other molecular measurements more sensitive to features present in the cancer cells. This is especially useful for samples with low tumor abundance.
Tissue dissociation converts a heterogeneous specimen into a cell preparation that can undergo selection based on size, density, or surface markers. This step makes the relevant differences accessible to physical or biological enrichment methods. Because the resulting cells may be used for genomic, transcriptomic, or molecular analysis, the process is paired with an emphasis on preserving material for downstream testing.
A typical workflow begins with heterogeneous tissue, followed by dissociation to produce a cell preparation. The sample then undergoes physical or biological selection using properties such as cell size, density, or surface-marker expression. The selected fraction is concentrated for subsequent analysis, while appropriate handling aims to preserve material needed for genomic, transcriptomic, or other molecular assays.
Researchers may use tumor enrichment when malignant cells are scarce or intermixed with surrounding normal cells. Increasing their representation can support mutation profiling, biomarker identification, and treatment-response studies by improving access to tumor-derived molecular information. The approach is therefore useful when the composition of the starting sample could otherwise limit detection or interpretation of cancer-related signals.
Tumor enrichment helps investigators obtain a more concentrated tumor-cell fraction from samples that also contain stromal and immune populations. That concentration supports molecular characterization of malignant cells and can contribute to studies of variation within tumors. By improving the tumor contribution to genomic or transcriptomic assays, the method provides a stronger basis for examining cancer-associated patterns in heterogeneous biological specimens.