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September 20, 2016
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We adapted a permeable microporous membrane insert to mimic the tumor microenvironment (TME). The model consists of a mixed cell culture, allows simplified generation of highly enriched individual cell populations without using fluorescent tagging or cell sorting, and permits studying intercellular communication within the TME under normal or stress conditions.
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Cite this Article
Domogauer, J. D., de Toledo, S. M., Azzam, E. I. A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication. J. Vis. Exp. (115), e54429, doi:10.3791/54429 (2016).
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