Vascular Extravasation
Vascular Extravasation - Related Videos
09:00
Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier
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Cited by 6 •
2017
Here, we describe a human blood-brain barrier model enabling to investigate lymphocyte transmigration into the central nervous system in vitro.
10:56
A Novel Three-dimensional Flow Chamber Device to Study Chemokine-directed Extravasation of Cells Circulating under Physiological Flow Conditions
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Cited by 7 •
2013
The three-dimensional flow chamber device is a novel in vitro technology for the quantitative and step-wise evaluation of the extravasation cascade of cells circulating under conditions of physiological shear stress. The device therefore fills a critical need for basic, preclinical, and clinical studies of cell migration.
07:31
Testing the Vascular Invasive Ability of Cancer Cells in Zebrafish (Danio Rerio)
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Cited by 19 •
2016
This method utilizes zebrafish embryos to efficiently test the vascular invasive ability of cancer cells. Fluorescent cancer cells are injected into the precardiac sinus or yolk sac of developing embryos. Cancer cell vascular invasion and extravasation is assessed via fluorescence microscopy of the tail region 24 to 96 hr later.
08:18
Engineered Vascularized Muscle Flap
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Cited by 6 •
2016
To date, thick tissue defects are typically reconstructed by applying autologous tissue flaps or engineered tissues. In this protocol, we present a new method for engineering vascularized tissue flap bearing an autologous pedicle, to serve as a substitute to autologous flaps.
11:10
Visualization of Vascular and Parenchymal Regeneration after 70% Partial Hepatectomy in Normal Mice
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Cited by 3 •
2016
Tools used for visualizing vascular regeneration require methods for contrasting the vascular trees. This film demonstrated a delicate injection technique used to achieve optimal contrasting of the vascular trees and illustrate the potential benefits resulting from a detailed analysis of the resulting specimen using µCT and histological serial sections.
FAQs
Adhesion molecules provide the attachment step that helps circulating tumor cells remain engaged with endothelial cells. Chemokine signals regulate cellular responses, while mechanical signals reflect forces associated with blood flow. Together, these inputs influence when and how tumor cells begin crossing the vascular barrier during metastatic dissemination.
Tumor cells encounter more than the endothelial cell layer itself. They must move through endothelial junctions and then across the underlying basement membrane before entering surrounding tissue. Examining both barriers helps clarify where passage may be regulated and how vascular structure affects the early stages of metastatic spread.
The same general movement across vascular endothelium is relevant to inflammation, immune surveillance, and cancer metastasis. In cancer research, this shared context helps investigators compare how different circulating cells interact with vascular barriers and determine how those interactions contribute to tissue entry, tumor dissemination, or immune monitoring.
The vascular microenvironment can affect what happens after tumor cells leave the bloodstream and enter surrounding tissue. Studying this relationship helps explain why certain vascular settings may support the establishment of tumor cells in distant organs. It also connects the extravasation step with later metastatic colonization rather than treating dissemination as a single event.
Experimental models of vascular extravasation support the evaluation of metastasis mechanisms and potential therapeutic targets. They allow researchers to examine how tumor-cell adhesion, responses to chemokine or mechanical signals, and passage across vascular barriers relate to metastatic behavior. These models therefore provide a framework for testing explanations of how primary tumors seed distant organs.
Endothelial barriers can affect the delivery and distribution of anticancer therapies. Investigating vascular extravasation provides context for understanding how substances encounter and move across vascular boundaries, while also revealing how the vascular microenvironment may influence access to surrounding tissue. This relevance links metastasis research with questions about therapeutic distribution in cancer.