Dimethylformamide
Dimethylformamide - Related Videos
13:37
Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library
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Cited by 5 •
2014
Peptide tertiary amides (PTAs) are a superfamily of peptidomimetics that include but are not limited to peptides, peptoids and N-methylated peptides. Here we describe a synthetic method which combines both split-and-pool and sub-monomer strategies to synthesize a one-bead one-compound library of PTAs.
07:07
Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology
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The synthesis of asymmetric species of ferrocene is challenging using solution techniques. This report focuses on the methods carried out to produce a ferrocene-biotin bioconjugate using facile and clean reactions accomplished via solid-phase synthesis. Incorporation of a thiolate moiety is shown to impart the ability for immobilization on gold surfaces.
03:59
UV-Vis Spectroscopy of Dyes - Instructor Prep
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2020
Source: Lara Al Hariri at the University of Massachusetts Amherst, MA, USA Preparation of Dye SolutionsExpand Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed. First, put on a lab coat, safety glasses, and nitrile gloves. Some solutions use hexane or dimethylformamide, so prepare those solutions in a fume hood. Prepare a 1 mM stock solution of fluorescein in water. Measure 0.0166 g of fluorescein...
06:49
Atom Transfer Radical Polymerization of Functionalized Vinyl Monomers Using Perylene as a Visible Light Photocatalyst
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Cited by 9 •
2016
A method for the atom transfer radical polymerization of functionalized vinyl monomers using perylene as a visible-light photocatalyst is described.
03:51
Fluorescent Functionalization of Virus-Like Particles via Disulfide Re-bridging
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2026
Source: Chen, Z., et al. Making Conjugation-induced Fluorescent PEGylated Virus-like Particles by Dibromomaleimide-disulfide Chemistry. J. Vis. Exp. (2018)This video demonstrates the fluorescent functionalization of virus-like particles by re-bridging reduced disulfides with a polyethylene glycol (PEG) linker, producing stable, trackable conjugates suitable for cellular imaging and targeted delivery in biomedical applications.
FAQs
The amide carbonyl gives DMF a strong dipole, creating pronounced charge separation within the molecule. This polarity helps DMF interact with dissolved species and supports its role as a medium for reactions that involve nucleophiles or charged intermediates. Consequently, the carbonyl group is central to how the solvent accommodates reactive components during substitution, coupling, and condensation processes.
Because DMF lacks an acidic hydrogen, it does not readily donate protons to dissolved reactants. This feature allows the solvent to solvate cations while limiting proton-transfer effects that could interfere with nucleophiles or charged intermediates. Its non-proton-donating behavior therefore helps maintain reaction conditions suited to transformations involving these reactive species.
DMF’s polarity influences how effectively reaction components, including charged species, remain accommodated in the reaction medium. By supporting cation solvation and interactions with polar reactants, it can provide conditions favorable to reactions involving nucleophiles and charged intermediates. The solvent’s behavior should therefore be considered alongside the reaction’s chemical requirements rather than treated as incidental.
Selection should account for DMF’s polarity, chemical reactivity, and volatility. Polarity determines its ability to dissolve and accommodate reaction components, while chemical reactivity means the solvent must be considered in the overall reaction environment. Volatility affects handling and processing. Evaluating these properties together helps researchers decide whether DMF suits a particular reaction or application.
DMF can serve as the reaction medium for substitution, coupling, and condensation reactions. Its polar environment supports systems containing nucleophiles and charged intermediates, while its ability to dissolve varied compounds helps bring reaction components into a usable solution. Researchers select it when these solvent characteristics align with the chemical requirements of the transformation.
The broad dissolving capacity of DMF extends beyond small-molecule reactions to polymer-related applications. It can dissolve many polymeric compounds, making it useful as a solvent in polymer processing and coatings. In these settings, its role is to provide a workable medium for polymer-containing formulations, supporting preparation and application of materials that require polymer dissolution.
DMF is used for analytical sample preparation because it can dissolve a wide range of organic, inorganic, and polymeric compounds, helping researchers prepare samples in a suitable solvent medium. Handling decisions should also consider its volatility and chemical reactivity. These properties influence how the solvent is selected, processed, and managed during laboratory work.