Cd Surface Markers
Cd Surface Markers - Related Videos
08:52
Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
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Cited by 3 •
2017
Liver injuries are accompanied by progenitor cell expansion that represents a heterogeneous cell population. Novel classification of this cellular compartment allows for the distinguishing of multiple subsets. The method described here illustrates the flow cytometry analysis and high purity isolation of various subsets that can be used for further assays.
03:20
Labeling Neural Cell Surface Markers with Azidosugar while Co-culturing with Endothelial Cells
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2025
This video demonstrates a protocol for co-culturing mouse brain endothelial cells and primary cortical stem cells and labeling the cell surface glycoproteins using Ac4ManNAz (azidosugar per-O-acetylated N-azidoacetylmannosamine). This method allows for selective enrichment and identification of cell surface proteins in primary cells.
10:32
Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
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Cited by 21 •
2014
Mouse embryonic fibroblast can be reprogrammed into induced pluripotent stem cells at low efficiency by the forced expression of transcription factors Oct-4, Sox-2, Klf-4, c-Myc. The rare intermediates of the reprogramming reaction are FACS isolated via labeling with antibodies against cell surface makers Thy-1.2, Ssea-1, and Epcam.
09:38
FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability
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Cited by 38 •
2011
Function-Spacer-Lipid (FSL) constructs allow the surface characteristics of living cells and virions to be modified without loss of vitality. The method requires only simple contact of an FSL construct solution with a cell/virion and spontaneous and stable surface incorporation occurs.
11:39
Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan
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Cited by 1 •
2019
Presented here is a protocol that combines an in vitro neural-endothelial co-culture system and metabolic incorporation of sialoglycan with bioorthogonal functional groups to expand primary neural stem and progenitor cells and label their surface sialoglycoproteins for imaging or mass-spectrometry analysis of cell surface markers.
FAQs
The measurement depends on a monoclonal antibody recognizing an extracellular epitope on a cell-surface molecule. Fluorescence associated with that binding indicates whether the marker is present and provides information about its abundance. Comparing these signals across cells helps researchers identify populations with different developmental states, rather than treating a culture as a uniform group.
Expression profiles built from multiple CD antigens provide a more informative way to distinguish related cell populations and stages of lineage commitment. A single marker may identify only one feature, whereas a combined profile can help separate progenitor cells, differentiated cells, and heterogeneous subpopulations. This makes marker panels useful for tracking changes during tissue formation and differentiation.
These approaches answer related but distinct questions. Fluorescence-based flow cytometry measures marker presence and abundance across cell populations, microscopy shows marker detection in a visual cellular context, and cell sorting uses marker-associated signals to isolate selected populations. Choosing among them depends on whether the goal is measurement, visualization, or recovery of cells for further study.
Changes in signals or culture conditions can alter the expression profile of CD antigens as cells progress toward different fates. Researchers can therefore compare profiles before and after a treatment or culture change to evaluate effects on lineage commitment and differentiation. The resulting shifts also help reveal changes in cellular heterogeneity within the experimental population.
A typical workflow begins by exposing cells to monoclonal antibodies directed against selected extracellular epitopes. The bound antibodies are then assessed through fluorescence-based flow cytometry or microscopy, or used to guide cell sorting. Researchers interpret the resulting marker presence, abundance, or expression profile to distinguish populations and examine developmental state.
They are useful when investigators need to follow lineage commitment, isolate progenitor or differentiated cells, or assess how experimental conditions influence cell fate. In studies of tissue formation and stem-cell differentiation, marker profiles provide a way to monitor changing populations. They also help evaluate cellular heterogeneity, which can reveal distinct developmental outcomes within the same culture.