Follicle Ovary Co-culture
Follicle Ovary Co-culture - Related Videos
10:41
Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
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Cited by 16 •
2015
This protocol describes the primary culture/co-culture of mouse ovarian tissue, using ovaries from neonatal mice and individual ovarian follicles from prepubertal mice. The culture techniques support development in a highly physiological manner, allowing investigation of the effect of extrinsic agents on the ovary, and of interactions between ovarian follicles.
10:31
Application of a Novel Hyaluronan Hydrogel for Three-Dimensional Follicle Culture and Methodology for Mouse Ovarian Follicle Cryopreservation
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Cited by 2 •
2025
This protocol describes a novel three-dimensional (3-D) culture model using a tyramine-linked hyaluronan hydrogel to encapsulate and culture preantral follicles from the mouse ovary. We also detail two approaches to ovarian follicle cryopreservation by vitrification.
08:15
Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries
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Cited by 11 •
2018
Here, we present a protocol to quantify follicles in cultured ovaries of young mice without serial sectioning. Using whole organ immunofluorescence and tissue clearing, physical sectioning is replaced with optical sectioning. This method of sample preparation and visualization maintains organ integrity and facilitates automated quantification of specific cells.
12:37
A Method for Ovarian Follicle Encapsulation and Culture in a Proteolytically Degradable 3 Dimensional System
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Cited by 80 •
2011
A new method for ovarian follicle encapsulation in a 3D fibrin-alginate interpenetrating network is described. This system combines structural support with proteolytic degradation to support the development of immature follicles to produce mature oocytes. This method may be applied to culture cell aggregates to maintain cell-cell contacts without limiting expansion.
07:13
Alginate Hydrogels for Three-Dimensional Organ Culture of Ovaries and Oviducts
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Cited by 43 •
2011
Culture of normal cells in their three-dimensional context represents an alternative method to study early events required for cellular transformation and tumorigenesis. This method is used to grow normal ovarian and oviductal cells to study early events in ovarian cancer formation.
FAQs
Preserved ovarian architecture allows follicles to remain in contact with surrounding stromal tissue rather than functioning as isolated units. These tissue relationships support extracellular-matrix interactions and local paracrine signaling, which can influence follicle growth and oocyte development. The resulting responses may more closely reflect how ovarian structure regulates reproductive cell development than systems lacking this tissue context.
Communication among oocytes, follicular cells, and ovarian stroma is central to the model. Follicular cells and surrounding tissue can participate in paracrine signaling, while the extracellular matrix provides structural interactions that help shape the local environment. Studying these relationships together helps connect follicle growth with changes in oocyte development and maturation.
The model retains multiple ovarian components that communicate with one another while exposed to hormonal cues. This makes it possible to examine how tissue context influences follicular responses rather than assessing hormonal effects only in separated cells. Such observations can clarify how ovarian organization contributes to folliculogenesis and the progression of oocyte development.
Researchers can use the system to examine folliculogenesis, follicle growth, oocyte development, and maturation-related responses. It also supports evaluation of oocyte competence, meaning the developmental quality or functional potential associated with successful reproductive use. Together, these outcomes help link ovarian tissue organization and signaling conditions with the development and function of reproductive cells.
The platform can support studies of fertility by showing how ovarian tissue conditions affect follicle and oocyte development. It can also be used to evaluate reproductive toxicants, with attention to their effects within a tissue environment that preserves cellular communication and matrix interactions. This provides context for interpreting how potentially harmful exposures may alter reproductive development.
In developmental biology, the model helps investigate how ovarian architecture regulates reproductive cell development and function. Its controlled culture setting also allows researchers to test conditions that may improve oocyte competence and inform tissue-based reproductive technologies. By connecting tissue organization with developmental outcomes, the approach can guide efforts to understand and support reproductive cell quality.