Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content.
You will only be able to see the first 20 seconds.

Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries
 

Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries

Article doi: 10.3791/57593
May 2nd, 2018

Summary May 2nd, 2018

Here, we present a protocol to quantify follicles in cultured ovaries of young mice without serial sectioning. Using whole organ immunofluorescence and tissue clearing, physical sectioning is replaced with optical sectioning. This method of sample preparation and visualization maintains organ integrity and facilitates automated quantification of specific cells.

Transcript

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter