Genome Editing
Genome Editing - Related Videos
08:31
Genome Editing
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2023
A well-established technique for modifying specific sequences in the genome is gene targeting by homologous recombination, but this method can be laborious and only works in certain organisms. Recent advances have led to the development of “genome editing”, which works by inducing double-strand breaks in DNA using engineered nuclease enzymes guided to target genomic sites by either proteins or RNAs that recognize specific sequences. When a cell attempts to repair this damage, mutations can be...
02:58
CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution
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2025
This video explains the concept of CRISPR-mediated cytosine base editors for inducing targeted nucleotide substitution.
08:51
Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks
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Cited by 30 •
2016
Transgenic manipulations and genome editing are critical for functionally testing the roles of genes and cis-regulatory elements. Here a detailed microinjection protocol for the generation of genomic modifications (including Tol2-mediated fluorescent reporter transgene constructs, TALENs, and CRISPRs) is presented for the emergent model fish, the threespine stickleback.
09:11
Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)
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Cited by 28 •
2012
The CompoZr Custom Zinc-Finger Nuclease (ZFN) Service enables precise genome editing in any organism or cell line at any locus defined by the user. This article describes the process for the design, manufacture, validation and implementation of the CompoZr Custom ZFN Service.
09:56
Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation
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2025
The efficiency of the Improved Genome Editing via Oviductal Nucleic Acids Delivery (I-GONAD) method is comparable to traditional microinjection, which requires zygote collection from donor females and transfer to pseudo-pregnant females. This protocol demonstrates its effectiveness by introducing CRISPR/Cas9-induced mutations into the ROSA26 locus on chromosome 6.
FAQs
The guide RNA determines which genomic sequence the Cas nuclease is directed toward by matching a corresponding DNA sequence. This targeting step connects a chosen gene or regulatory region to a specific molecular intervention. In developmental studies, selecting different targets allows researchers to examine whether particular sequences influence gene function, enhancer activity, cell fate, or tissue formation.
The cell’s repair route influences the type of genetic change that follows a targeted DNA break. End joining can produce gene disruptions, whereas template-guided repair can support defined sequence changes when a repair template guides the process. Comparing these outcomes helps researchers choose between disrupting gene function and testing a particular sequence alteration during development.
Enhancers are genomic regions whose alteration can help reveal how regulatory DNA contributes to gene activity during development. Editing these sequences allows researchers to connect a specific regulatory element with developmental outcomes rather than examining only the gene’s coding sequence. This approach supports analysis of how genetic information influences cell fate and tissue formation.
Researchers can alter a targeted gene and then examine the resulting developmental consequences in cells or organisms. A disruption may reveal whether that gene contributes to processes such as cell fate or tissue formation, while a defined sequence change can test the effect of a more specific genetic alteration. These comparisons connect DNA sequence changes with biological traits.
Mutant embryos provide a developmental context in which the consequences of a targeted genetic alteration can be examined as cells and tissues form. By generating embryos carrying gene disruptions or defined sequence changes, researchers can investigate how altered genetic information affects developmental processes. This application makes genome editing useful for linking gene function to visible developmental outcomes.
Engineered model organisms allow researchers to study the developmental effects of selected genetic changes in an organismal setting. Genome editing can connect altered sequences with gene function, cell fate, and tissue formation, while also supporting investigation of developmental disorders. These models extend findings beyond isolated genetic sequences by placing them within broader developmental processes.