Lymph Gland Mounting
Lymph Gland Mounting - Related Videos
11:49
Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae
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Cited by 5 •
2016
Drosophila and mammalian hematopoietic systems share many common features, making Drosophila an attractive genetic model to study hematopoiesis. Here we demonstrate dissection and mounting of the major larval hematopoietic organ for immunohistochemistry. We also describe methods to assay various larval hematopoietic compartments including circulating hemocytes and sessile crystal cells.
04:20
Larval Lymph Gland Dissection: Isolating the Drosophila Hemocyte-Producing Organ
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2023
This video describes the Drosophila lymph gland—the primary site of hematopoiesis in the fly larva—and shows an example protocol to dissect and mount the organ for immunohistochemistry and fluorescent imaging.
09:22
Sectioning Mammary Gland Whole Mounts for Lesion Identification
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Cited by 13 •
2017
We developed a method to successfully remove, process, section, and stain, for histopathological evaluation, mammary tissue that had originally been fixed on slides as whole mounts. This method may promote the collection and evaluation of mammary gland whole mounts in reproductive and developmental test guideline studies.
11:02
Quantifying Branching Density in Rat Mammary Gland Whole-mounts Using the Sholl Analysis Method
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Cited by 29 •
2017
Mammary gland development in the rodent has typically been evaluated using descriptive assessments or by measuring basic physical attributes. Branching density is an indicator of mammary development that is difficult to quantify objectively. This protocol describes a reliable method for the quantitative assessment of mammary gland branching characteristics.
10:02
High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes
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Cited by 6 •
2015
This protocol describes the application of high-frequency ultrasound (HFUS) for imaging mouse cervical lymph nodes. This technique optimizes visualization and quantification of cervical lymph node morphology, volume and blood flow. Image-guided biopsy of cervical lymph nodes and processing of lymph tissue for histological evaluation is also demonstrated.
FAQs
Fixation helps preserve the dissected gland during preparation, while staining makes cellular features or molecular markers detectable for microscopy. Together, these steps determine whether the resulting mount reveals tissue organization, progenitor populations, differentiated blood-cell zones, or signaling-related patterns. Their combined effect allows developmental changes in hematopoietic tissues to be examined in a preserved specimen.
Orientation places the gland in a consistent position beneath the coverslip, allowing its cellular architecture and distinct developmental regions to be viewed clearly. A suitable orientation helps relate progenitor populations, differentiated blood-cell zones, and areas of cell division to one another. This spatial organization is essential when comparing tissue patterns across developmental stages or experimental conditions.
Mounted glands can preserve the spatial relationship between cells and molecular markers, enabling fluorescence microscopy to show where signaling pathways are associated with developing hematopoietic regions. Researchers can examine these patterns alongside progenitor and differentiated cell populations. This makes the preparation useful for studying how blood-cell formation is organized and regulated during organ development.
The preparation begins with dissection of the lymph gland, followed by fixation to preserve the tissue. The specimen is then stained, positioned in mounting medium, and placed beneath a coverslip for microscopic examination. This sequence produces a stable preparation in which cellular architecture and selected molecular markers can be imaged using fluorescence microscopy.
This method is useful when researchers need to examine how hematopoietic tissues change as an organ develops. A mounted gland can reveal progenitor populations, differentiated blood-cell zones, cell division, and signaling patterns within the same preserved specimen. It therefore supports investigations of tissue organization and the regulation of blood-cell formation during development.
Fluorescence imaging can make molecular markers and cellular regions visible within the mounted gland. Researchers may use these images to identify progenitor and differentiated populations, examine cell division, and compare signaling patterns across developmental tissue zones. When analyzed quantitatively, the images can provide evidence about how hematopoiesis is spatially organized and regulated.