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JoVE Journal
Developmental Biology

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Quantifying Branching Density in Rat Mammary Gland Whole-mounts Using the Sholl Analysis Method
 

Quantifying Branching Density in Rat Mammary Gland Whole-mounts Using the Sholl Analysis Method

Article doi: 10.3791/55789
July 12th, 2017

Summary July 12th, 2017

Mammary gland development in the rodent has typically been evaluated using descriptive assessments or by measuring basic physical attributes. Branching density is an indicator of mammary development that is difficult to quantify objectively. This protocol describes a reliable method for the quantitative assessment of mammary gland branching characteristics.

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