Transgenic Embryos
Transgenic Embryos - Related Videos
17:39
Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes
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Cited by 56 •
2007
In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue fever. The techniques for correctly preparing microinjection needles, dessicating embryos, and performing microinjection are demonstrated.
04:29
Ex Vivo Culture and Imaging of Oculomotor Slices from Transgenic Mouse Embryos
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2025
This video demonstrates the ex vivo culture and imaging of oculomotor slices from transgenic mouse embryos. The embryos are isolated and embedded in agarose. Using a vibratome, slices containing the oculomotor nuclei and eyes are obtained. The slices are maintained in culture and imaged using a fluorescence microscope.
09:10
Generation of Transgenic Hydra by Embryo Microinjection
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Cited by 34 •
2014
Stably transgenic Hydra are made by microinjection of plasmid DNA into embryos followed by random genomic integration and asexual propagation to establish a uniform line. Transgenic Hydra are used to track cell movements, overexpress genes, study promoter function, or knock down gene expression using RNAi.
05:40
Tol2 Transposon-Mediated Zebrafish Transgenesis: A Procedure to Generate Transgenic Zebrafish Following the Coinjection of Tol2 System Into Fertilized Zebrafish Embryos
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2025
In this video, we demonstrate a procedure to generate a stable zebrafish transgenic line constitutively expressing a fluorescent voltage indicator protein following the co-injection of the Tol2 transposon system comprising the transposase mRNA and Tol2 transposon construct encoding the fluorescent voltage indicator.
10:36
In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences
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Cited by 2 •
2017
Live tracking of individual WT retinal progenitors in distinct genetic backgrounds allows for the assessment of the contribution of cell non-autonomous signaling during neurogenesis. Here, a combination of gene knockdown, chimera generation via embryo transplantation and in vivo time-lapse confocal imaging was utilized for this purpose.
FAQs
An introduced gene affects development only when its regulatory control permits appropriate expression. In that setting, researchers can relate the transgene’s presence to changes observed as the embryo develops. This connection helps test whether a specific gene contributes to developmental processes rather than merely being present in embryonic DNA.
Transgenic embryos can connect genetic changes with cell lineage decisions by showing how altered genetic material relates to the cells and tissues that emerge. Researchers examine developmental patterns and resulting traits, then use those observations to evaluate gene function. This makes the embryos useful for tracing how genetic information influences organized development.
Regulatory sequences influence when and where introduced genetic material is expressed. Comparing developmental outcomes under different regulatory control can therefore help distinguish a gene’s general presence from its activity in particular developmental contexts. In developmental biology, that distinction supports analysis of tissue patterning and responses involving developmental pathways.
A typical workflow begins by introducing a transgene into an egg, zygote, or early embryo through microinjection or genome editing. The resulting embryo is then examined as development proceeds, with attention to genetic material, expression under suitable control, cell behavior, tissue patterning, or observable traits. These observations connect manipulation with outcome.
Researchers choose this approach when they need to test a relationship between a genetic alteration and development. The resulting system can provide evidence about gene function, cell lineage decisions, tissue patterning, and developmental pathways. Its value comes from comparing the introduced genetic change with observable developmental outcomes in the embryo.
In disease modeling, transgenic embryos provide an experimental setting for examining how a genetic change affects development. They can also support tests of biological hypotheses by linking altered DNA to developmental pathways and observable traits. This combination makes them relevant when researchers need to study disease-related effects in the context of early development.