Human Stem Cells
Human Stem Cells - Related Videos
10:24
Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
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Cited by 26 •
2011
Despite recent advancements in genetic modification, transfection of human embryonic stem cells (HESCs) remains a capricious process. To our knowledge, systematic and efficient methods to transfect human induced pluripotent stem cells (iPSCs) have not been reported. Here, we describe robust protocols to efficiently transfect and nucleofect human iPSCs.
10:33
Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells
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Cited by 24 •
2014
Neural crest (NC) cells derived from human pluripotent stem cells (hPSC) have great potential for modeling human development and disease and for cell replacement therapies. Here, a feeder-free adaptation of the currently widely used in vitro differentiation protocol for the derivation of NC cells from hPSCs is presented.
08:05
Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research
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Cited by 3 •
2016
Here is a protocol to grow pluripotent stem cells (PSC) and neural stem cells (NSC) in an enclosed cell culture system that permits maximum sterility and reproducibility, replacing the traditional biosafety cabinet and incubator. This equipment meets clinical good manufacturing practice (cGMP) and clinical good lab practice (cGLP) guidelines.
15:42
Xenotransplantation of Human Stem Cells into the Chicken Embryo
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Cited by 31 •
2010
In this paper we present a method for transplanting human stem cells into various regions of the central nervous system of the chicken embryo. This provides an in vivo model for assessing the proliferation and differentiation of various types of human stem cells in embryonic tissue environments.
09:02
Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)
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Cited by 45 •
2013
This protocol describes the development, expansion, and in vivo imaging of NK cells derived from hESCs and iPSCs.
FAQs
Internal gene-regulatory networks help maintain the balance between continued cell division and retention of an unspecialized state. Their activity interacts with external signals, so stem cells do not operate independently of their surroundings. Studying this relationship allows researchers to examine how cells remain available for future developmental decisions while avoiding premature commitment to a particular lineage.
External signals provide information that can shift cells from maintaining stemness toward differentiation, the process of acquiring specialized characteristics. The resulting response depends on the stem-cell type and the signals present in its environment. In developmental biology, manipulating these influences in laboratory cultures helps investigators study how cell-fate decisions lead toward particular lineages and tissues.
Embryonic and induced pluripotent stem cells are especially useful for modeling early development, tissue formation, and cell-fate decisions in laboratory cultures. Adult stem cells provide complementary insight into tissue maintenance and repair. Comparing these sources helps researchers connect early developmental processes with the continuing biological demands of established tissues.
Laboratory cultures allow researchers to examine how stem cells divide, retain stemness, and begin producing cells associated with specific lineages. These observations can reveal relationships between regulatory networks, external signals, and developmental outcomes. In developmental biology, such systems provide a controlled way to investigate tissue formation and the sequence of decisions that shape developing cell populations.
Stem-cell-based systems can reproduce selected aspects of human development or tissue biology in laboratory cultures, creating models for studying disease-related processes. They also support drug evaluation by providing cellular contexts in which responses can be investigated. These applications extend stem-cell research beyond basic development and help connect cellular mechanisms with potential therapeutic research.
Stem-cell research is relevant to regenerative therapies because the cells can be studied in relation to tissue formation, maintenance, and repair. Embryonic and induced pluripotent models inform how specialized lineages arise, while adult stem-cell models address tissue upkeep and restoration. Together, these systems provide scientific context for evaluating whether cell-based approaches may support damaged or diseased tissues.