Hydrodynamic Dielectrophoresis
Hydrodynamic Dielectrophoresis - Related Videos
10:13
A Microfluidic-based Hydrodynamic Trap for Single Particles
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Cited by 14 •
2011
In this article, we present a microfluidic-based method for particle confinement based on hydrodynamic flow. We demonstrate stable particle trapping at a fluid stagnation point using a feedback control mechanism, thereby enabling confinement and micromanipulation of arbitrary particles in an integrated microdevice.
10:38
Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
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Cited by 19 •
2013
Contactless dielectrophoresis (cDEP) achieves sorting and enrichment of particles via their intrinsic dielectric properties. Fluidic electrode channels replace metallic electrodes traditional to DEP, suiting cDEP to non-damaging sterile characterization and sorting of biological particles. We demonstrate how to prepare a cDEP microdevice and conduct cell characterization and sorting experiments.
12:54
Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice
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Cited by 26 •
2014
In vivo transfection of naked DNA by hydrodynamic gene delivery introduces genes into the tissue of an animal with minimal inflammatory response. Sufficient amounts of gene product are generated such that gene function and regulation as well as protein structure and function can be analyzed.
11:12
Protocol for Relative Hydrodynamic Assessment of Tri-leaflet Polymer Valves
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Cited by 5 •
2013
There has been renewed interest in developing polymer valves. Here, the objectives are to demonstrate the feasibility of modifying a commercial pulse duplicator to accommodate tri-leaflet geometries and to define a protocol to present polymer valve hydrodynamic data in comparison to native and prosthetic valve data collected under near-identical conditions.
03:48
Hydrodynamic Gene Delivery: A Technique for In Vivo Transfection of Exogenous DNA in Murine Hepatocytes via Tail Vein Injection
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2025
This video describes the technique of genetic in vivo transfection of murine hepatocytes by hydrodynamic tail vein injection. This transfection system generates a long-term expression of the transgene in the hepatocytes.
FAQs
Frequency changes the induced dipole response of a suspended object, so the resulting dielectrophoretic force can vary with the object’s properties. In a nonuniform field, that frequency-dependent force competes with fluid drag and redirects particles. Adjusting frequency therefore helps create distinct trajectories, providing a basis for differentiating and manipulating cells or particles without labels.
Sheath or carrier flows establish where particles enter and travel within the channel, rather than leaving their initial positions uncontrolled. This hydrodynamic positioning places objects in a defined region of the nonuniform electric field, where dielectrophoretic force and drag can act predictably. The result is improved trajectory control for focusing, separation, or enrichment.
Different suspended objects do not necessarily follow the same path because their properties influence the induced dipole force and its balance with fluid drag. Under the same channel flow and electric-field conditions, those differences can redirect objects along separate trajectories. This behavior supports label-free separation and particle characterization.
Particles are introduced into a microfluidic channel while sheath or carrier flows position them in a defined region. A nonuniform electric field is then used alongside the controlled flow, and particle paths are assessed as force and drag produce different trajectories. This sequence can support focusing, sorting, enrichment, or alignment.
HDEP is suited to tasks that require label-free handling of cells or suspended particles, including sorting, concentration, alignment, and characterization. In biomedical diagnostics, it can provide a microfluidic route for manipulating cell populations; in particle analysis, it supports differentiated trajectories. These uses connect physical separation behavior with lab-on-a-chip engineering.
Flow-based operation gives HDEP potential for continuous processing rather than requiring every object to be handled individually. Its contactless operation may also support gentle handling, which is relevant when working with cells. Together, these features make the method attractive for integrated microfluidic systems used in diagnostics and other analytical workflows.