Pmma-assisted Transfer
Pmma-assisted Transfer - Related Videos
04:54
Solvent Bonding for Fabrication of PMMA and COP Microfluidic Devices
0 Views •
Cited by 30 •
2017
Solvent bonding is a simple and versatile method for fabricating thermoplastic microfluidic devices with high quality bonds. We describe a protocol to achieve strong, optically clear bonds in PMMA and COP microfluidic devices that preserve microfeature details, by a judicious combination of pressure, temperature, an appropriate solvent, and device geometry.
02:13
Centrifugation-Assisted Mitochondrial Transfer to Glioblastoma Stem Cells
0 Views •
2025
In this video, a method is demonstrated to enable the transfer of fluorescently labeled mitochondria into glioblastoma stem cells (GSCs) through centrifugation. The successful uptake and functional integration of the donor mitochondria are confirmed by detecting the fluoresce using confocal microscopy.
02:59
Agarose-Embedded Electroporation-Assisted Gene Transfer in Mouse Cortical Interneuron Progenitors
0 Views •
2025
Source: Denaxa, M., el al., Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains. J. Vis. Exp. (2019)This video demonstrates the procedure for injecting a plasmid vector carrying the gene of interest into interneuron progenitors in an embryonic brain slice, followed by electroporation for plasmid entry and gene expression. These modified progenitors can later be used for treating brain disorders.
09:42
Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples
0 Views •
2016
The fabrication protocol of a dipole-assisted solid phase extraction microchip for the trace metal analysis is presented.
09:09
Layer-by-layer Synthesis and Transfer of Freestanding Conjugated Microporous Polymer Nanomembranes
0 Views •
Cited by 3 •
2015
In this paper we describe the interfacial synthesis of conjugated microporous polymers (CMP) on sacrificial substrates, and the dissolution of the substrate for the preparation of freestanding CMP nanomembranes. In addition, we will describe how the fragile nanomembranes can be transferred to other substrates.
FAQs
PMMA acts as a temporary mechanical support for fragile thin films, membranes, and two-dimensional materials during relocation. Coating the material before substrate separation helps keep the layer together while the original substrate is selectively etched or otherwise separated. Afterward, the polymer can be removed, leaving the transferred layer on its new surface.
The selective separation step releases the supported layer from its original substrate without requiring direct growth at the destination. This expands integration options when the target substrate is incompatible with the starting material’s growth conditions. In engineering, that distinction supports the assembly of layered devices and heterostructures on deliberately chosen surfaces.
The target surface becomes part of the engineered interface being examined. After relocation, researchers can study how that interface affects material performance within a layered structure or heterostructure. Consequently, transfer supports both the handling of delicate materials and investigations of performance changes associated with substrate and interface selection.
A typical workflow starts by coating the thin film, membrane, or two-dimensional material with PMMA. The original substrate is then selectively etched or separated, allowing the supported layer to be placed on a target surface. Finally, the PMMA is dissolved in a suitable solvent, completing relocation and removing the temporary support.
Dissolving PMMA after placement removes the temporary support only after the relocated material has reached its target surface. This sequence separates the handling function of the polymer from the final device or interface structure. The result is a transferred layer that can remain integrated with the selected substrate without the support layer.
The method is useful when researchers need to relocate a fragile layer, combine materials into a layered device, or assemble a heterostructure on a substrate that does not support direct growth. It also provides a way to examine how selected interfaces influence material performance, linking materials processing with device integration and interface engineering.