Plant Contaminant Transport

Plant contaminant transport is the movement of chemical pollutants through plants and between plant tissues, an important process in environmental fate and ecosystem health. Contaminants may enter through roots, leaves, or stems and then move with water through the xylem or with sugars through the phloem; their transport depends on solubility, binding to soil, plant physiology, and chemical properties. Studying these pathways helps predict contaminant accumulation in roots, shoots, and edible tissues, assess risks to food webs and human health, and evaluate phytoremediation strategies that use plants to remove, stabilize, or transform pollutants in contaminated environments.

Plant Contaminant Transport - Related Videos

Research

JoVE Journal - Environment

A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems

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Cited by 5 •

2014

A whole cell bioreporter assay with Burkholderia sartisoli RP037-mChe was developed to detect fractions of an organic contaminant (i.e., fluorene) available for bacterial degradation after active transport by mycelia bridging air-filled pores in a water unsaturated model system.

Research

JoVE Journal - Genetics
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Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

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Cited by 15 •

2016

Existing methods for the modification of plants have limited applicability. The novel peptide-derived technology proposed here promises both simplicity and efficiency in the introduction of exogenous protein or genes into the desired intracellular compartments of intact plants.

Research

JoVE Journal - Genetics
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Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

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Cited by 26 •

2018

Here, we present a protocol for tracing genomic DNA (gDNA) contamination in RNA samples. The presented method utilizes primers specific for the internal transcribed spacer region (ITS) of ribosomal DNA (rDNA) genes. The method is suited for reliable and sensitive detection of DNA contamination in most eukaryotes and prokaryotes.

Research

JoVE Journal - Environment
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Two-Dimensional Visualization and Quantification of Labile, Inorganic Plant Nutrients and Contaminants in Soil

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Cited by 13 •

2020

This protocol presents a workflow for sub-mm 2D visualization of multiple labile inorganic nutrient and contaminant solute species using diffusive gradients in thin films (DGT) combined with mass spectrometry imaging. Solute sampling and high-resolution chemical analysis are described in detail for quantitative mapping of solutes in the rhizosphere of terrestrial plants.

Research

JoVE Journal - Biology
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Transmitting Plant Viruses Using Whiteflies

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Cited by 41 •

2013

Improving our understanding of and our ability to manage many of the insect-transmitted plant viruses requires the use of the vector. Insect transmission of plant viruses is a tritrophic interaction, and as such requires the manipulation of insects, virus, and plant. Vectors must be reared in large numbers and manipulated in such a way as to insure high rates of transmission to test plants. The basics of rearing and manipulating the whitefly, Bemisa tabaci (Hemiptera: Aleyrodidae), a vector of...

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