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A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems
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JoVE Journal Environment
A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems

A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems

DOI:
10.3791/52334-v

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13:16 min

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December 24, 2014

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, , ,
1Department of Environmental Microbiology,Helmholtz Centre for Environmental Research – UFZ, 2Department of River Ecology,Helmholtz Centre for Environmental Research – UFZ

Chapters

  • 00:05Title
  • 01:59Preparation of Dishes, Slides, Media and Cultures
  • 03:21Microcosm Mounting
  • 07:11Confocal Laser Scanning Microscopy
  • 08:21Image Analysis and Chemical Quantification
  • 10:30Results: Detection of Fluorene Available for Bacterial Degradation Using Bioreporter Assay
  • 12:22Conclusion

Summary

Automatic Translation

Automatic Translation

A whole cell bioreporter assay with Burkholderia sartisoli RP037-mChe was developed to detect fractions of an organic contaminant (i.e., fluorene) available for bacterial degradation after active transport by mycelia bridging air-filled pores in a water unsaturated model system.

Tags

Keywords: BioavailabilityContaminant TransportUnsaturated SoilMyceliaBioreporterPAHFluoreneBurkholderiaPythium UltimumConfocal Laser Scanning Microscopy

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