In Vivo Screening
In Vivo Screening - Related Videos
07:03
Large Scale Zebrafish-Based In vivo Small Molecule Screen
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Cited by 17 •
2010
Zebrafish has emerged as a powerful in vivo platform for phenotype-based drug screens and chemical genetic analysis. Here, we demonstrate a simple, practical method for large-scale screening of small molecules using zebrafish embryos.
08:44
Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening
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Cited by 7 •
2013
Genes can be manipulated during development of the cortex or the hippocampus of the rat via in utero electroporation (IUE) at E16, to enable fast and targeted modifications in neuronal connectivity for later studies of behavior or neuropathology in adult animals. Postnatal in vivo imaging for control of IUE success is performed by bioluminescence of activating co-transfected luciferase.
09:16
Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System
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Cited by 36 •
2015
Ex vivo ERG can be used to record electrical activity of retinal cells directly from isolated intact retinas of animals or humans. We demonstrate here how common in vivo ERG systems can be adapted for ex vivo ERG recordings in order to dissect the electrical activity of retinal cells.
09:44
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
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2015
Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.
08:50
Genetic Screens
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2023
Genetic screens are critical tools for defining gene function and understanding gene interactions. Screens typically involve mutating genes and then assessing the affected organisms for phenotypes of interest. The process can be “forward”, where mutations are generated randomly to identify unknown genes responsible for the phenotypes, or it can be “reverse”, where specific genes are targeted for mutation to observe what phenotypes are produced.Here, JoVE reviews various types of genetic...
FAQs
The living context preserves interactions among tissues and biological systems that isolated cells may not reproduce. As a result, a genetic change can be evaluated through organism-level consequences rather than through a response in one cellular setting alone. This broader context is especially relevant when the outcome involves development, behavior, disease, or survival.
Researchers compare organisms carrying defined genetic changes, or exposed to different experimental conditions, and examine differences in their observed phenotypes. These comparisons help associate particular gene activities with traits or biological responses. Evaluating multiple outcomes across conditions can also show whether an effect is linked to development, behavior, disease, or survival.
Phenotypes provide the observable evidence used to connect a genetic change with organism-level biology. Measurements may reflect development, behavior, disease, or survival, depending on the research question. Interpreting these outcomes in the organism helps researchers assess how gene activity relates to integrated biological responses rather than to an isolated molecular or cellular observation.
A typical workflow begins by introducing or selecting defined genetic variants, gene perturbations, or candidate interventions in living organisms. Researchers then observe the resulting phenotypes under specified experimental conditions and compare those outcomes across organisms or groups. The comparisons identify candidates or genetic changes that produce effects relevant to the study question.
The approach can reveal effects on development, behavior, disease, and survival, as well as broader links between gene activity and organism-level traits. Because responses are measured in living organisms, the results reflect interactions among tissues and biological systems. These outcomes help prioritize genetic changes or candidate interventions for further research.
Researchers apply it to gene-function studies, disease modeling, target validation, and prioritizing candidates for additional investigation. It is useful when the research requires evidence from an intact organism, including how genetic changes influence development, behavior, disease, or survival. In this context, the method connects genetic perturbations with physiologically integrated outcomes.