Adhesive Coated Plate
Adhesive Coated Plate - Related Videos
03:10
Micro-Parallel Plate Flow Chamber Model for Studying Bacterial Adhesion to Human Endothelial Cells
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2026
Source: Claes, J., et al, In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions. J. Vis. Exp. (2015).This video demonstrates a method for studying host-pathogen interactions using a micro-parallel plate flow chamber seeded with human primary endothelial cells. Following endothelial cell activation by an ionophore and perfusion with fluorescently labeled bacteria, the cells release von Willebrand factor (VWF), which binds bacterial adhesins under shear...
02:47
In Vitro Parallel-Plate Flow Model to Study Bacterial Adhesion on a Tissue Biopsy
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2026
Source: Ditkowski, B., et al. An In Vitro Model of a Parallel-Plate Perfusion System to Study Bacterial Adherence to Graft Tissues. J. Vis. Exp. (2019).This video demonstrates the method of using a parallel-plate flow chamber to perfuse fluorescently labeled bacteria over vascular graft tissues, enabling controlled analysis of bacterial adhesion and early pathogenesis under shear flow conditions.
01:14
Adhesion
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2019
Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules. Capillary action is a result of water’s adhesive tendencies. When a narrow glass...
04:46
Resorption Pit Assay: An In Vitro Technique to Quantify Calcium Resorption Activity of Mature Osteoclasts using Calcium Phosphate-Coated Culture Plates
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2025
In this video, we describe an in vitro osteoclastic resorption assay on calcium phosphate-coated cell culture plates cultured with human peripheral blood mononuclear cell-derived osteoclasts. The osteoclasts form a characteristic actin ring on the calcium phosphate-coated matrix of the cell culture plate and degrade the matrix within the actin ring region to release calcium and phosphate ions, resulting in the formation of distinct resorption pits within the degraded matrix.
02:41
B Cell Activation on an Antigen-Coated Coverslip
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2025
This video demonstrates B cell activation in vitro. The process involves antigen interaction with B cell receptors, leading to cytoskeleton remodeling, immune synapse formation, lysosome movement, antigen internalization, processing, and subsequent presentation on MHC molecules for B cell activation.
FAQs
Retention can arise from electrostatic attraction, hydrophobic forces, or specific binding between the treated surface and deposited biological material. These interactions may involve proteins, cells, antigens, or antibodies. Because the target remains localized in the well, subsequent incubation and analysis can proceed with less loss during handling and washing steps.
Stable retention keeps deposited material in the intended well while assay conditions change. During washing, localized targets are less likely to be removed, supporting more consistent analysis. During incubation, maintaining the position of antigens, antibodies, proteins, or cells helps preserve the interaction being studied and can improve the reliability of signal detection.
The treated surface can interact with several classes of biological targets, including proteins, cells, antigens, and antibodies. Electrostatic attraction, hydrophobic forces, and specific binding provide distinct ways to support attachment. This range allows the same general plate format to accommodate different experimental purposes, from immune-binding measurements to cell-adhesion studies.
A typical workflow deposits the selected biological material into treated wells, allows attachment or retention during incubation, and then uses washing steps before analysis. The retained material remains available for subsequent binding or detection measurements. This sequence supports consistent localization of targets throughout the handling stages described for immunology and infection assays.
Applications include enzyme-linked immunosorbent assays, antibody-binding studies, antigen-binding studies, pathogen detection, and cell-adhesion experiments. In these settings, the plate provides a surface for retaining the relevant biological material while researchers perform incubation, washing, and signal analysis. The resulting measurements can support evaluation of immune responses or characterization of infectious agents.
In immunology, retained antigens or antibodies can support measurements of binding and immune responses. In infection research, localized biological material can be used in pathogen detection or studies of infectious agents. The treated wells also support cell-adhesion experiments, extending the format beyond soluble binding measurements to cellular interactions.
These plates support analysis of binding behavior, immune-response measurements, pathogen detection, and cell attachment. Their treated wells help keep targets localized through incubation and washing, which contributes to assay consistency and signal detection. Researchers can therefore compare or characterize biological interactions involving antigens, antibodies, proteins, cells, or infectious agents.