Blood Culture Isolation
Blood Culture Isolation - Related Videos
06:03
Isolation and Characterization of Microvesicles from Peripheral Blood
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Cited by 74 •
2017
Extracellular vesicles present in blood have been suggested as novel biomarkers for various diseases. Here, we present a protocol for the isolation of large plasma membrane-derived microvesicles from peripheral blood samples and their subsequent analysis by conventional flow cytometry and Western Blotting.
10:46
Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice
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Cited by 89 •
2010
Here, we describe a protocol for isolation and culture of murine pulmonary endothelial cells. This method comprises mechanic and enzymatic lung tissue dissociation as well as a 2-step purification process using anti-PECAM-1 and anti-ICAM-2 antibodies conjugated to magnetic beads, which produces a pure endothelial cell population of mostly microvascular origin.
07:26
Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood
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Cited by 16 •
2017
The goal of this protocol is to isolate endothelial progenitor cells from umbilical cord blood. Some of the applications include using these cells as a biomarker for identifying patients with cardiovascular risk, treating ischemic diseases, and creating tissue-engineered vascular and heart valve constructs.
03:18
COVID-19 / Coronavirus Outbreak: How to take blood cultures
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2020
In pandemic times, medical staff is becoming a key resource in fighting the infection. To achieve the best medical care, relevant techniques and procedures have to be taught to medical staff while reducing the risk of infection. Blood cultures play a key role in an effective anti-infective therapy regime. As COVID-19 patients develop bacterial superinfections in the damaged pulmonary tissue, using the correct technique to obtain blood culture samples and following anti-infective therapy are...
11:00
Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood
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Cited by 74 •
2015
This protocol allows for the reliable generation and characterization of blood outgrowth endothelial cells (BOECs) from a small volume of adult peripheral blood. BOECs can be used as a surrogate for endothelial cells from patients with vascular disorders and as a substrate for the generation of induced pluripotent stem cells.
FAQs
Nutrient media supplies the growth conditions needed for microorganisms present in a blood sample to multiply enough for detection. Its role is therefore not merely to hold the specimen: it supports recovery before the organism is isolated and identified. The resulting culture evidence can then be paired with susceptibility testing.
Using both aerobic and anaerobic incubation broadens the conditions under which bloodstream microorganisms can be recovered. The distinction matters because organisms may differ in their relationship to oxygen, so a single incubation environment could fail to support recovery of some organisms. Selecting these paired conditions improves the chance that clinically relevant growth will be detected.
A detectable change in a culture bottle is an important signal, but it is only the beginning of interpretation. Laboratory work must proceed to organism isolation and identification, followed by antimicrobial susceptibility testing. This sequence converts evidence of microbial growth into information about the organism involved and the treatment options it may support.
Blood Culture Isolation must be interpreted with attention to contamination, because recovery of a microorganism does not automatically establish true infection. In immunology and infection research, comparing the culture finding with host immune responses helps clarify whether the result fits bacteremia, fungemia, sepsis, or another invasive disease. This context supports more meaningful conclusions.
Following collection, the blood sample is inoculated into culture bottles containing nutrient media, then incubated under aerobic or anaerobic conditions. Detectable growth prompts recovery of the organism for isolation and identification. Antimicrobial susceptibility testing follows as an additional stage, producing data that connect laboratory recovery with treatment selection and resistance surveillance.
Beyond individual diagnosis, the method supports several connected uses. Results can guide targeted antimicrobial treatment, contribute to surveillance of antimicrobial resistance, and help investigators examine sepsis and other invasive diseases. In infection research, combining organism recovery with host-response information strengthens interpretation of how circulating microorganisms relate to disease and immune activity.