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JoVE Journal
Biology

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Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood
 

Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood

Article DOI: 10.3791/53384 11:00 min
December 23rd, 2015

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Summary December 23rd, 2015

This protocol allows for the reliable generation and characterization of blood outgrowth endothelial cells (BOECs) from a small volume of adult peripheral blood. BOECs can be used as a surrogate for endothelial cells from patients with vascular disorders and as a substrate for the generation of induced pluripotent stem cells.

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