Cell Phenotyping
Cell Phenotyping - Related Videos
07:07
Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea
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Cited by 8 •
2016
Primary cilia influence various signaling pathways. The mammalian cochlea is ideal for examining planar cell polarity (PCP) signaling. Cilia dysfunction affects cochlear outgrowth, cellular patterning and hair cell orientation, readouts of PCP. Our goal is to analyze PCP signaling in mouse cochlea via phenotypic analysis, immunohistochemistry and scanning electron microscopy.
07:35
A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva
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Cited by 5 •
2017
The exposed normal ocular surface consists of cornea and conjunctiva. Epithelial cells, goblet cells and immune cells are present in the conjunctiva. Here, a non-invasive, technique of impression cytology is described using an impression cytology device and flow cytometry to analyze immune cells in the conjunctiva.
05:42
A Barcoding Assay for Phenotypic and Functional Characterization of Immune Cells
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2025
This video highlights a single-cell proteomic method that employs heavy-metal-conjugated antibodies for distinct cell barcoding. Subsequently, immunostaining and mass cytometry are applied to evaluate immune phenotypic and functional alterations in human whole blood-derived immune cells.
03:14
Phenotyping EBV-Infected Cell Lines Using Flow Cytometry
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2026
Source: Xu, C., et al. An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection. J. Vis. Exp. (2018).This video demonstrates the phenotyping of EBV-infected T and NK cell lines using flow cytometry, highlighting the preparation, antibody staining, and analysis steps to identify lineage-specific surface markers. It shows how marker profiles confirm distinct T-cell and NK-cell populations.
03:38
Preparation of a Micropatterned Substrate to Study Schwann Cell Phenotypes
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2025
The video describes the preparation of a micropatterned substrate to study cell phenotype specification. A protein-coated micropatterned PDMS stamp is used to transfer the micropattern on a PDMS-coated coverslip. The coverslip is then treated with a surfactant to hinder cell adhesion at the unwanted surfaces. Cells are then added to settle in the pattern and incubated for further analysis.
FAQs
Combining measurements from surface and intracellular markers with functional readouts helps separate immune subsets that may share some physical features. Surface markers support identification of cell populations, whereas intracellular cytokines and viability measurements add evidence about activity and survival. This multi-parameter view is especially useful when infection changes cellular characteristics.
Flow cytometry supports measurement of multiple surface or intracellular markers, cytokine production, and viability. Microscopy provides another way to examine cellular characteristics, while single-cell analysis helps characterize individual cellular states within a broader sample. Using these approaches together can provide complementary evidence about immune responses and pathogen-associated changes.
Changes in marker expression, cytokine production, viability, and other measured features can indicate that cells have entered different functional states. Researchers can use these patterns to track immune activation, differentiation, or exhaustion rather than treating all cells as equivalent. Comparing these readouts across conditions helps reveal how immune responses evolve during infection or other interventions.
Separating pathogen-infected cells from immune cells is essential for interpreting host-pathogen interactions. A measured change may reflect altered behavior in the infected population, an immune response directed against it, or both. Distinguishing these cellular groups allows researchers to examine how pathogens modify cell state while also tracking the response of surrounding immune populations.
A basic workflow begins by selecting cellular features relevant to the research question, such as surface or intracellular markers, cytokine production, viability, or functional state. Researchers then apply an appropriate measurement approach, including flow cytometry, microscopy, or single-cell analysis, and compare the resulting profiles across tissues, conditions, or disease stages.
Cell phenotyping is useful when researchers need cellular-level evidence for infection diagnosis, vaccine evaluation, or immunotherapy development. It can also characterize immune responses and pathogen effects across tissues and disease stages. These applications rely on comparing marker patterns, functional measurements, and cell states to determine how an intervention or infection changes the cellular response.
Measuring the same cellular features across tissues or disease stages enables researchers to follow shifts in immune subsets, pathogen-infected cells, and functional states over time or location. Such comparisons can reveal differences in activation, differentiation, exhaustion, viability, or cytokine production. The resulting profiles help connect cellular changes with the progression or treatment context of infection.