Cfse Staining
Cfse Staining - Related Videos
10:57
Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry
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2023
Source: Perchet Thibaut1,2,3, Meunier Sylvain1,2,3, Sophie Novault4, Rachel Golub1,2,3 1 Unit for Lymphpoiesis, Department of Immunology, Pasteur Institute, Paris, France 2 INSERM U1223, Paris, France 3 Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France 4 Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute, Paris, France The cell cycle is a universal process of life. During the cell cycle, a cell undergoes...
09:49
Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells
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Cited by 39 •
2010
Many infections elicit a strong CTL response, but occasionally, the quantity of responding cells does not correlate to control of the pathogen1. One measure of CTL quality is their ability to kill specifically2. CFSE labeling of target cells can be used to investigate this CTL response quality in vivo3,4.
04:10
The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation
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Cited by 214 •
2010
CFSE covalently labels long-lived intracellular molecules with the fluorescent dye, carboxyfluorescein. As such, when a CFSE-labeled cell divides, its progeny have half the amount of fluorescence, which can thereby be used to assess cell division. This article describes the procedures typically used for labeling mouse lymphocytes with CFSE.
11:54
Identification and Isolation of Slow-Dividing Cells in Human Glioblastoma Using Carboxy Fluorescein Succinimidyl Ester (CFSE)
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Cited by 18 •
2012
This video protocol demonstrates the application of the fluorescent dye carboxyfluorescein succinimidyl ester (CFSE) for the identification and separation of different sub-populations of cells in human glioblastoma based on frequency of cell division.
04:34
A CFSE-Based Assay to Monitor Skin Dendritic Cell Migration to Draining Lymph Nodes in Infected Mice
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2025
This video demonstrates an assay for monitoring dendritic cell migration following footpad infection with Mycobacterium bovis Bacille Calmette-Guérin BCG in mice. Upon bacterial infection, activated skin DC cells are labeled with a cell-permeable carboxyfluorescein diacetate succinimidyl ester (CFSE) dye. After a suitable interval for DC migration to the popliteal draining lymph node, the node cells are isolated and subjected to immunostaining for DC-specific surface markers. Identification of...
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Once inside the cell, the membrane-permeable dye is converted into a reactive form that binds intracellular amines. When a labeled cell divides, the resulting fluorescence is distributed between the two daughter cells. Repeated divisions therefore produce stepwise decreases in signal, allowing proliferation to be distinguished from an undivided population.
Each distinct fluorescence peak represents cells with a related dilution of the original label after division. Examining the sequence of peaks allows researchers to quantify how many cells proliferated and estimate their division history. This provides more information than simply recording whether a sample contains fluorescent or nonfluorescent cells.
Flow cytometry detects the separate fluorescence peaks generated as labeled cells divide. Because the analysis can reveal distinct responding cell populations, researchers can quantify proliferation within the relevant immune-cell group rather than relying only on a bulk sample measurement. The resulting pattern connects fluorescence loss with the extent of the immune response.
The general workflow begins by labeling cells with CFSE, allowing the labeled population to undergo the immune or infection-related response being studied, and then measuring fluorescence by flow cytometry. Researchers interpret the resulting intensity distribution and division-associated peaks to quantify proliferation and estimate how extensively cells have divided.
CFSE staining is useful when researchers need to measure immune-cell proliferation during infection or another immune response. It can support studies of lymphocyte activation, antigen-specific responses, and changes caused by pathogens, vaccines, or immunomodulatory treatments. These applications make the technique relevant for comparing how different conditions influence cellular immune activity.
By tracking fluorescence changes in labeled immune cells, the method can reveal which cell populations respond with proliferation during an antigen-specific challenge. Flow-cytometric patterns help identify responding populations and estimate their division history. In infection studies, this information can be used to examine how pathogens, vaccines, or treatments affect antigen-driven cellular responses.