Mouse Infection
Mouse Infection - Related Videos
09:01
Chronic Salmonella Infected Mouse Model
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Cited by 12 •
2010
Establish a chronic bacterial infected mouse model with persistent Salmonella typhimurium colonization in intestine for 27 weeks.
03:16
Processing of Infected Tissues for Bacterial Enumeration in a Mouse Co-Infection Model
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2026
Source: Lenhard, A., et al. A Mouse Model for the Transition of Streptococcus pneumoniae from Colonizer to Pathogen upon Viral Co-Infection Recapitulates Age-Exacerbated Illness. J. Vis. Exp. (2022)This video demonstrates the processing of lungs from a mouse co-infected with a pathogenic bacterium and a virus. Mincing, homogenizing, and plating the lung tissue allows quantification of bacterial load, indicating bacterial spread to the lungs and disease severity.
09:24
Transurethral Induction of Mouse Urinary Tract Infection
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Cited by 33 •
2010
This video will demonstrate methods to transurethrally induce mouse urinary tract infections and quantify the extent of resulting infections.
10:44
Influenza A Virus Studies in a Mouse Model of Infection
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Cited by 38 •
2017
Influenza A viruses (IAVs) are important human respiratory pathogens. To understand the pathogenicity of IAVs and to perform preclinical testing of novel vaccine approaches, animal models mimicking human physiology are required. Here, we describe techniques to evaluate IAV pathogenesis, humoral responses and vaccine efficacy using a mouse model of infection.
03:42
Establishing a Mouse Model of Vaginal Group B Streptococcus Infection
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2026
Source: Patras, K. A., Doran, K. S. A Murine Model of Group B Streptococcus Vaginal Colonization. J. Vis. Exp. (2016)This video demonstrates the preparation and vaginal inoculation of a hormone-treated female mouse with Group B Streptococcus to model infection. It explains how to culture and prepare the bacteria, administer the inoculum into the vaginal lumen, and ensure successful infection.
FAQs
A defined administration route standardizes how the pathogen is introduced, allowing researchers to compare infection development and host responses across experimental groups. Because the route is controlled, changes in pathogen burden, tissue condition, innate immune signaling, cytokine production, or adaptive immunity can be interpreted in relation to a consistent exposure procedure rather than an uncontrolled mode of infection.
These measurements provide complementary evidence about disease and immunity. Pathogen burden indicates how much infectious material is present, while tissue changes show effects on organs or other examined sites. Innate immune signaling and cytokine production reveal how the host detects and responds to infection. Together, the readouts connect pathogen presence with tissue consequences and immune activation.
Antibody- and T-cell-mediated responses help researchers determine how adaptive immunity develops during infection. Examining these responses can indicate whether immunity is associated with protection or with harmful effects. This distinction is important because an immune response may limit the pathogen, contribute to tissue damage, or provide evidence about mechanisms that could be targeted by vaccines or other interventions.
A study generally begins by selecting the pathogen and defining the administration route. Researchers then monitor infection development and assess outcomes such as pathogen burden, tissue changes, innate immune signaling, cytokine production, and antibody- or T-cell-mediated responses. The selected measurements depend on whether the experiment is examining host-pathogen interactions, immune mechanisms, or a potential intervention.
These models allow researchers to examine whether a vaccine, antimicrobial treatment, antiviral treatment, or other intervention changes infection-related outcomes. Investigators can compare pathogen burden, tissue changes, immune signaling, cytokine production, and adaptive responses between experimental conditions. Findings may identify protective effects, harmful immune mechanisms, or evidence that supports advancing an intervention toward clinical research.
Mouse infection studies connect pathogen behavior with the host response in an experimental setting. They support investigation of bacteria, viruses, parasites, and fungi while enabling analysis of innate signaling, cytokines, antibodies, and T cells. This broad scope helps researchers study host-pathogen interactions, identify mechanisms of protection or harm, and relate immune findings to disease intervention strategies.