Multi-species Coculture
Multi-species Coculture - Related Videos
10:05
The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture
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Cited by 91 •
2015
Here, we present a protocol to coculture primary cells, tissue models and punch biopsies in a microfluidic multi-organ chip for up to 28 days. Human dermal microvascular endothelial cells, liver aggregates and skin biopsies were successfully combined in a common media circulation.
02:51
Multi-species Conserved Sequences
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2021
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms. Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
07:09
Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms
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Cited by 28 •
2014
The goal of this methods paper is to describe the use of a microfluidic system for the development of multi-species biofilms that contain species typically identified in human supragingival dental plaque. Methods to describe biofilm architecture, biofilm viability, and an approach to harvest biofilm for culture-dependent or culture-independent analyses are highlighted.
09:23
Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion
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Cited by 19 •
2016
Understanding the malignant behavior of cancer requires creating accurate models of how tumor cells interact with components of the tumor microenvironment, such as macrophages. Here we describe two methods to study glioblastoma cell interaction with tumor associated macrophages and microglia where the effect on glioblastoma invasion is assessed.
03:24
Coculturing of Retinal Ganglion Neurons with Olfactory Ensheathing Glia
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2025
This video demonstrates the procedure to isolate retinal ganglion neurons (RGNs) from a rat's retina and coculture them with olfactory ensheathing glia (OEG) cells. This method allows for studying the neuro-regenerative potential of OEGs after neural injury.
FAQs
Interactions can occur through metabolite exchange, competition for nutrients, and changes in signaling pathways. One species may alter the environment in ways that affect another species’ growth or survival, while shared conditions allow researchers to observe these effects together. Monitoring community changes can reveal relationships that remain hidden when each microbe is studied separately.
Nutrient competition can limit the growth or survival of particular members, whereas metabolite exchange may support or modify neighboring species. These opposing effects help determine how a community develops under defined conditions. Examining both processes gives researchers a more complete view of microbial relationships than measuring the behavior of one species without its potential partners.
When host or immune cells encounter a community rather than a single microbe, signals generated by multiple species can influence the cellular response. Microbes may also modify one another before or during that interaction, changing the context detected by immune cells. This makes coculture useful for examining immune responses to complex microbial communities in infection research.
The shared environment should be defined and controlled so that observed changes can be attributed to interactions among the cultured species and host cells, rather than to uncontrolled culture variation. Researchers then monitor effects on community behavior, growth, survival, or signaling. Consistent conditions are especially important when comparing mixed cultures with single-species or host-cell systems.
It is particularly valuable when commensal organisms may alter pathogen behavior or when immune cells respond differently to a microbial community than to one organism. The approach can expose interaction-dependent infection mechanisms and host-microbe relationships that single-species cultures may miss. It therefore provides broader context for studying pathogen behavior in the presence of other community members.
These models support studies of infection mechanisms, host-microbe relationships, antimicrobial activity, and therapeutic strategies. Researchers can monitor how community members influence one another’s growth and survival, how pathogens behave alongside commensals, and how immune cells respond to the resulting community. Such observations can help evaluate effects that would not appear in isolated cultures.