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Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms
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JoVE Journal Bioengineering
Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms

Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms

DOI:
10.3791/52467-v

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07:09 min

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December 01, 2014

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, , ,
1Department of Epidemiology, School of Public Health,The University of Michigan, 2Centre for Oral Health Research, School of Dental Sciences,Newcastle University

Chapters

  • 00:05Title
  • 02:06Preparation of Pooled Human Saliva as a Bacteria-positive Inoculum
  • 02:51Growth and Live-dead Staining of Biofilms in a Microfluidic Chip
  • 05:57Results: Live-dead Staining of Oral Cavity Biofilms Grown in Microfluidic Chips
  • 06:43Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this methods paper is to describe the use of a microfluidic system for the development of multi-species biofilms that contain species typically identified in human supragingival dental plaque. Methods to describe biofilm architecture, biofilm viability, and an approach to harvest biofilm for culture-dependent or culture-independent analyses are highlighted.

Tags

Keywords: High-throughputIn VitroMicrofluidic SystemMulti-species BiofilmsOral BiofilmsHuman SalivaSupragingival Dental PlaqueConfocal Laser Scanning Microscopy3D Biofilm ReconstructionBiofilm ArchitectureBiofilm ViabilityStreptococcusNeisseriaVeillonellaGemellaPorphyromonasBiofilm Cell HarvestingDNA ExtractionData Analysis

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