Use of a High-throughput <em>In Vitro</em> Microfluidic System to Develop Oral Multi-species Biofilms

Derek S. Samarian; Nicholas S. Jakubovics; Ting L. Luo; Alexander H. Rickard

doi:10.3791/52467

Journal

/

Bioengineering

/

Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms

Use of a High-throughput <em>In Vitro</em> Microfluidic System to Develop Oral Multi-species Biofilms

JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Bioengineering

Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms

Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms

13,401 Views

•

07:09 min

•

December 01, 2014

DOI:

10.3791/52467-v

DOI:

10.3791/52467-v

•

07:09 min

December 01, 2014

•

6 Views

Derek S. Samarian¹, Nicholas S. Jakubovics², Ting L. Luo¹, Alexander H. Rickard¹

¹Department of Epidemiology, School of Public Health,The University of Michigan, ²Centre for Oral Health Research, School of Dental Sciences,Newcastle University

Summary

The goal of this methods paper is to describe the use of a microfluidic system for the development of multi-species biofilms that contain species typically identified in human supragingival dental plaque. Methods to describe biofilm architecture, biofilm viability, and an approach to harvest biofilm for culture-dependent or culture-independent analyses are highlighted.