15o-water Pet
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01:16
Other Nuclides: 31P, 19F, 15N NMR
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2024
Many organic, inorganic, and biological molecules contain spin-half nuclei such as nitrogen-15, fluorine-19, and phosphorus-31. As a result, NMR studies of these nuclei have found extensive applications in chemical and biological research. While fluorine-19 and phosphorous-31 have high natural abundances (100%) and positive gyromagnetic ratios, nitrogen-15 has a low natural abundance and a negative gyromagnetic ratio. However, nitrogen-15 is still preferred over nitrogen-14 (which has a high...
08:44
Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis
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Cited by 2 •
2020
A microplot design for 15N tracer research is described to accommodate multiple in-season plant and soil sampling events. Soil and plant sample collection and processing procedures, including grinding and weighing protocols, for 15N analysis are put forth.
10:35
Simultaneous PET/MRI Imaging During Mouse Cerebral Hypoxia-ischemia
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Cited by 4 •
2015
The method presented here uses simultaneous positron emission tomography and magnetic resonance imaging. In the cerebral hypoxia-ischemia model, dynamic changes in diffusion and glucose metabolism occur during and after injury. The evolving and irreproducible damage in this model necessitates simultaneous acquisition if meaningful multi-modal imaging data are to be acquired.
10:53
Functional Imaging of Brown Fat in Mice with 18F-FDG micro-PET/CT
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Cited by 37 •
2012
A method of functional imaging of mouse brown adipose tissue (BAT) is described in which cold-stimulated uptake of 18F-Fluorodeoxyglucose (FDG) in BAT is non-invasively assessed with a standardized micro-PET/CT protocol. This method is robust and sensitive to detect differences in BAT activities in mouse models.
10:47
Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging
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Cited by 7 •
2015
The bioorthogonal inverse electron demand Diels-Alder cycloaddition has been harnessed to create an effective and modular pretargeted PET imaging strategy for cancer. In this protocol, the steps of this methodology are described in the context of a model system employing the colorectal cancer targeted antibody huA33 and a 64Cu-labeled radioligand.
FAQs
The method follows how oxygen-15-labeled water enters and moves through tissue after intravenous administration. As the tracer undergoes positron decay, the resulting annihilation photons are detected by the PET scanner. Analysis of the tracer’s changing concentration over time, known as dynamic tracer kinetics, allows researchers to estimate blood flow in specific tissue regions.
Because the tracer is freely diffusible, its passage through tissue closely reflects delivery by the bloodstream rather than remaining confined to the vascular space. This property supports quantitative assessment of regional perfusion, including differences in blood supply between brain regions. The resulting measurements can help characterize how circulation changes across tissue or experimental conditions.
Oxygen-15’s very short half-life allows measurements to focus on rapid physiological changes. It also supports repeat assessments under different conditions, enabling comparison of regional blood flow across successive measurements. This temporal flexibility is particularly valuable when investigators examine changing brain function, disease-related perfusion, or blood-flow responses associated with treatment.
A study administers the labeled water intravenously and records the tracer as it passes through tissue. The PET scanner detects annihilation photons produced during positron decay, while the acquisition captures changes over time. Those dynamic data are then analyzed with tracer kinetics to estimate perfusion in selected regions, especially regional cerebral blood flow.
Dynamic tracer kinetics provide quantitative information about how tracer delivery changes over time, rather than only showing where activity is present. In 15O-water PET, this analysis supports estimates of blood flow for specific regions. Researchers can therefore compare perfusion between areas or conditions and evaluate treatment-related changes in circulation.
Its applications include investigating cerebrovascular disease, studying brain function, and characterizing tumor perfusion. The technique also helps examine treatment-related changes in blood flow. Because it provides quantitative regional measurements and can capture rapid physiological changes, it is suited to research and clinical investigation where perfusion patterns are central to interpretation.