Airborne Allergen Sensitization
Airborne Allergen Sensitization - Related Videos
05:37
Analyzing Basophil Activation in the Presence of a Test Allergen using Flow Cytometry
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2025
In this video, we demonstrate the test to evaluate basophil activation in the presence of a test allergen using flow cytometry.
03:31
A Microfluidic Cartridge-Based Detection System for Allergen-Specific Immunoglobulin E
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2025
This video demonstrates a protocol for detecting serum-specific immunoglobulin E using a microfluidic cartridge-based chemiluminescence system. Serum IgEs interact with distinct allergens immobilized in the reaction zone of the cartridge, which are subsequently detected with peroxidase-conjugated anti-IgE antibodies and a chemiluminescent substrate, allowing simultaneous detection of multiple allergen-specific IgEs.
10:42
Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10
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Cited by 8 •
2016
Here, we present a protocol to quantify allergen-loaded particles by flow cytometry. Ambient particulate matter particles may act as carriers of adsorbed allergens. We show here that flow cytometry, a method widely used to characterize suspended solids >0.5 µm in diameter, can be used to measure these allergen-loaded particles.
06:34
A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma
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Cited by 4 •
2017
This work describes a protocol that uses a component-resolved approach to study sensitization to grass pollen allergens in a cohort of patients from southern China with allergic rhinitis and/or asthma.
08:05
Murine Model of Allergen Induced Asthma
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Cited by 51 •
2012
Experimental mouse models of allergic asthma offer new possibilities for studying disease pathogenesis and developing new therapeutics. These models are well suited to measuring factors governing the allergic immune response, airway inflammation, and pulmonary pathophysiology.
FAQs
A later exposure can activate mast cells that have already bound allergen-specific immunoglobulin E, or IgE. These cells then release inflammatory mediators, producing the biological response associated with allergic symptoms. This priming effect explains why the initial exposure establishes immune readiness, while subsequent encounters can trigger manifestations in susceptible respiratory tissues.
Antigen-presenting cells begin the adaptive immune sequence by activating allergen-specific T cells and B cells. This cellular coordination leads to production of IgE directed against the inhaled substance. The resulting specificity allows the immune system to recognize the same allergen during later exposures and connect that recognition with mast-cell activation.
The relevant exposures include biological particles commonly present in indoor or outdoor air. Examples include pollen, mold spores, dust mite particles, and animal dander. Their sources differ, but each can provide an inhaled allergen capable of initiating the immune pathway. Identifying the likely source helps connect environmental exposure with respiratory symptoms.
Clinical evaluation begins with an exposure history, which examines the relationship between symptoms and contact with suspected airborne substances. Allergy testing can provide additional evidence of allergen-specific immune reactivity. Together, these approaches help clinicians identify relevant triggers and distinguish potentially important exposures from the many particles encountered in everyday environments.
Recognizing the relevant airborne trigger supports several management strategies. Patients and clinicians can develop avoidance measures aimed at reducing contact with the suspected allergen, while pharmacological treatment addresses the associated condition. Allergen-specific immunotherapy is another option informed by the identified sensitization, linking diagnostic information with a more targeted management plan.
This immune process can contribute to allergic rhinitis, asthma, and other respiratory conditions. The clinical relevance therefore extends beyond identifying an isolated environmental sensitivity: it may help explain nasal or lower-airway disease and support decisions about testing, exposure reduction, medication, or allergen-specific immunotherapy. The same sensitization framework can inform evaluation across respiratory presentations.