Cd73 Cd90 Cd105
Cd73 Cd90 Cd105 - Related Videos
12:40
Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
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Cited by 91 •
2016
Herein we describe methods for the dissection of fetal and maternal tissues from human term placenta, followed by isolation and expansion of mesenchymal stem/stromal cells (MSC) from these tissues.
09:47
Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs
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Cited by 3 •
2016
This protocol describes an isolation technique for obtaining primary lung resident mesenchymal stromal cells from rats, through the use of enzymatic digestion, density gradient separation, plastic adherence and CD146+ magnetic bead selection.
11:29
HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells
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Cited by 5 •
2016
The protocol described here represents an easy and reproducible method that employs reverse phase high-performance liquid chromatography (RP-HPLC) to measure purine metabolism on chronic lymphocytic leukemia (CLL) cells cultured under different conditions.
08:28
Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy
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Cited by 21 •
2017
This protocol describes a method to image resident tumor-infiltrating CD8 T cells labeled with fluorescently coupled antibodies within human lung tumor slices. This technique permits real-time analyses of CD8 T cell migration using confocal microscopy.
11:44
Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells
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Cited by 3 •
2016
Here, we present a novel humanized mouse liver model generated in Alb-toxin receptor mediated cell knockout (TRECK)/SCID mice following the transplantation of immature and expandable human hepatic stem cells.
FAQs
CD73 contributes to extracellular nucleotide processing by converting AMP outside the cell into adenosine. This enzymatic step gives the marker functional significance beyond its presence on the cell surface. In stromal-cell studies, researchers can therefore relate CD73 expression to investigations of immunomodulation, tissue repair, and the biological behavior of enriched cell populations.
These markers are complementary, not interchangeable. CD73 reports ecto-5′-nucleotidase activity, CD90 is associated with cell adhesion and signaling, and CD105 participates as an accessory receptor in transforming growth factor-beta pathways. Reading the three together gives researchers different biological dimensions for characterizing stromal populations, rather than relying on a single surface feature.
Expression patterns across CD73, CD90, and CD105 can help researchers characterize and distinguish cell populations in a sample or culture. Adding lineage-negative markers provides further context for interpreting that pattern. Together, these measurements support evaluations of cell identity and enrichment, making the panel useful for determining whether a culture reflects the intended stromal-cell population.
Flow cytometry and immunostaining provide two established ways to assess CD73, CD90, and CD105 expression. Researchers can use these approaches to examine marker profiles across cell populations and to support evaluations of cell identity, enrichment, and culture quality. The resulting measurements are especially useful when the three markers are interpreted as a panel.
Adding lineage-negative markers gives the positive CD73, CD90, and CD105 profile additional context. Rather than evaluating the three markers in isolation, researchers can compare their expression with the absence of lineage-associated markers. This combined strategy supports cell-population distinction and helps assess whether enrichment and culture quality are consistent with the intended stromal-cell population.
The marker set is relevant to studies of tissue repair, regenerative medicine, immunomodulation, and the therapeutic potential of stromal cell populations. In these settings, expression profiling helps researchers characterize and enrich the cells under study and monitor culture quality. It therefore connects basic cell-surface analysis with investigations of how stromal populations may be used in biomedical research.