Dead Cell Elimination
Dead Cell Elimination - Related Videos
10:47
Quantification of Proliferative and Dead Cells in Enteroids
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Cited by 2 •
2020
The presented protocol uses flow cytometry to quantify the number of proliferating and dead cells in cultured mouse enteroids. This method is helpful to evaluate the effects of drug treatment on organoid proliferation and survival.
03:49
Near-Infrared Photoimmunotherapy for Targeted Cell Elimination in Mixed 3D Cultures
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2025
This video demonstrates a technique for selectively removing specific cells from a mixed 3D cell culture through near-infrared photoimmunotherapy. In this method, targeted cells are accurately identified using an antibody conjugated with an NIR photoabsorber. Subsequent irradiation with infrared light induces cell death, effectively eliminating the targeted cells from the 3D culture.
05:18
Selective Cell Elimination from Mixed 3D Culture Using a Near Infrared Photoimmunotherapy Technique
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Cited by 21 •
2016
Eliminating specific cells without damaging other cells is extremely difficult, especially in established tissue, yet there is an urgent need for a cell elimination method in the tissue engineering field. Here, we present a method for specific cell elimination from a mixed 3D cell culture using near infrared photoimmunotherapy (NIR-PIT).
09:48
In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages
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Cited by 7 •
2021
Neurodegenerative diseases are associated with dysregulated microglia functions. This article outlines an in vitro assay of phagocytosis of neuroblastoma cells by iPSC-macrophages. Quantitative microscopy readouts are described for both live-cell time-lapse imaging and fixed-cell high-content imaging.
01:26
Enhanced Elimination of Poison
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2023
Poison can be effectively removed from the gastrointestinal (GI) tract through various decontamination procedures. Antidotes serve a crucial role in counteracting the effects of poison by inhibiting enzymes responsible for producing harmful drug metabolites. In some cases, these toxic metabolites can be neutralized by endogenous cosubstrates, which are maintained at specific concentrations to prevent interaction with cellular macromolecules and subsequent cell death. Renal excretion is the...
FAQs
Phosphatidylserine functions as an eat-me signal on the surface of cells undergoing apoptosis. Its display helps attract phagocytes and directs them toward the appropriate cellular targets. Because recognition occurs before engulfment and digestion, this signal provides a useful mechanistic point for investigating whether damaged cells are identified and removed efficiently in tissues.
Efferocytosis allows phagocytes to engulf and digest cellular remains after the dying cell has packaged its contents and signaled for removal. This organized handling limits unnecessary exposure of cellular material within the tissue. The result supports removal without the same degree of inflammatory disruption associated with uncontrolled release from damaged cells.
Efficient clearance helps tissues remove cellular material while maintaining a controlled local environment. In medicine, this process is relevant to immune tolerance because defective removal can contribute to autoimmunity and persistent inflammation. Studying recognition, engulfment, and digestion therefore helps connect cellular waste handling with the preservation of healthy immune responses.
A typical conceptual analysis follows the process from cellular death or irreparable damage through packaging of cell contents, display of eat-me signals, phagocyte attraction, engulfment, and digestion. Researchers can then consider whether removal remains coordinated and whether inflammation is limited. This sequence links individual cellular events with tissue-level maintenance and injury resolution.
When damaged or expired cells are not removed effectively, their persistence can interfere with normal tissue maintenance and injury resolution. The overview connects defective clearance with chronic inflammation, autoimmunity, and infection-related damage. These outcomes make clearance efficiency an important medical context for understanding why some tissues fail to return to a healthy state.
Medical research can use this process to identify whether disease reflects inadequate clearance or abnormal cell survival. Potential therapeutic directions include restoring removal mechanisms or targeting cells that persist inappropriately. This framework is especially relevant to tumor persistence, while also helping researchers consider how defective elimination may sustain inflammation, autoimmunity, or infection-related tissue damage.