Journal
/
Neuroscience
/
In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages

In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages

DOI:
10.3791/62217-v

•

09:48 min

•

February 14, 2021

•

, , , ,
1James Martin Stem Cell Facility, Sir William Dunn School of Pathology,University of Oxford, 2Alzheimer’s Research UK Oxford Drug Discovery Institute, Nuffield Department of Medicine Research Building,University of Oxford, 3UK Dementia Research Institute,Cardiff University

Chapters

  • 00:04Introduction
  • 00:41Preparation of Dead SH-SY5Ys
  • 02:03Labeling of Dead SH-SY5Ys with pH-Sensitive Red Fluorescent Dye
  • 03:18Staining of iPSC-Macrophages
  • 04:50Fixed-Cell High-Content Imaging
  • 07:28Results: Imaging, Optimization and Validation of Phagocytosis Assay
  • 09:16Conclusion

Summary

Automatic Translation

Automatic Translation

Neurodegenerative diseases are associated with dysregulated microglia functions. This article outlines an in vitro assay of phagocytosis of neuroblastoma cells by iPSC-macrophages. Quantitative microscopy readouts are described for both live-cell time-lapse imaging and fixed-cell high-content imaging.

Tags

Keywords: In VitroQuantitative Imaging AssayPhagocytosisDead Neuroblastoma CellsIPSC-macrophagesNeurodegenerative DiseaseSH-SY5Y CellsCell DissociationFixationPH-sensitive DyeFluorescent DyeMacrophage MediaHoechst 33342

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62217?article_type=v

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
check_url/62217?article_type=v

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae
check_url/62217?article_type=v

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors
check_url/62217?article_type=v

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
check_url/62217?article_type=v

In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation
check_url/62217?article_type=v

In Vitro Phagocytosis of Myelin Debris by Bone Marrow-Derived Macrophages
check_url/62217?article_type=v

Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells
check_url/62217?article_type=v

A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes
check_url/62217?article_type=v

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening
check_url/62217?article_type=v

Quantitative Measurement of Intrathecally Synthesized Proteins in Mice

Read Article